Cyclophilin B Antibody

Images

 
Western Blot: Cyclophilin B Antibody [NBP1-85358] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: Cyclophilin B Antibody [NBP1-85358] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: Cyclophilin B Antibody [NBP1-85358] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Cyclophilin B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:LKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPIB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Simple Western 8 ug/mL
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cyclophilin B Protein (NBP1-85358PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-85358 in the following applications:

Publications
Read Publications using NBP1-85358.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24339976)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyclophilin B Antibody

  • Cyclophilin B
  • cyclophilin-like protein
  • CYPB
  • CYPBEC 5.2.1.8
  • CYP-S1
  • MGC2224
  • OI9MGC14109
  • peptidyl-prolyl cis-trans isomerase B
  • peptidylprolyl isomerase B (cyclophilin B)
  • PPIase B
  • PPIB
  • Rotamase B
  • S-cyclophilin
  • SCYLP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-85358
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
H00064175-B01P
Species: Hu
Applications: WB
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP1-94105
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-57062
Species: Hu
Applications: WB, ICC/IF
NBP2-14016
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
MAB3777
Species: Hu, Mu, Rt
Applications: WB
AF972
Species: Hu
Applications: WB, Simple Western, IHC
H00002288-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P, IP
AF4094
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-38604
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-58529
Species: Hu
Applications: ICC/IF
NBP1-85360
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF4174
Species: Hu, Mu, Rt
Applications: WB, Simple Western
NBP1-87929
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB600-408
Species: Hu, Mu, Rt, Po, Bv, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-83889
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88766
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13794
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Cyclophilin B Antibody (NBP1-85358)(2)

Review for Cyclophilin B Antibody (NBP1-85358) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Simple Western.

Reviews using NBP1-85358:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Cyclophilin B NBP1-85358
Enlarge
reviewed by:
Rob Wilpan
Simple Western Mouse 04/21/2017
View

Summary

ApplicationSimple Western
Sample Testedmouse liver lysate
SpeciesMouse
LotA106997

Comments

CommentsUsed NBP1-85358 anti-Cyclophilin B antibody with B6 Mouse Liver Lysate @ 1mg/ml because of the lack of sensitivity of another antibody. Runs as 28 kDa on the 12 to 230 kDa kit. Antibody titration of 1:6.25 to 1:200 is shown.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyclophilin B Antibody (NBP1-85358) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cyclophilin B Products

Bioinformatics Tool for Cyclophilin B Antibody (NBP1-85358)

Discover related pathways, diseases and genes to Cyclophilin B Antibody (NBP1-85358). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cyclophilin B Antibody (NBP1-85358)

Discover more about diseases related to Cyclophilin B Antibody (NBP1-85358).
 

Pathways for Cyclophilin B Antibody (NBP1-85358)

View related products by pathway.

PTMs for Cyclophilin B Antibody (NBP1-85358)

Learn more about PTMs related to Cyclophilin B Antibody (NBP1-85358).
 

Research Areas for Cyclophilin B Antibody (NBP1-85358)

Find related products by research area.

Blogs on Cyclophilin B

There are no specific blogs for Cyclophilin B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Rob Wilpan
04/21/2017
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol PPIB
Entrez