Cyclophilin B Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data ...read more
Genetic Strategies: Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. ...read more
Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Cyclophilin B Antibody [NBP1-85358] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human thyroid gland shows high expression.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human prostate shows weak granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Cyclophilin B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPIB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Simple Western 8 ug/mL
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Cyclophilin B antibody validated for Simple Western from a verified customer review.
Control Peptide
Cyclophilin B Protein (NBP1-85358PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-85358 in the following applications:

Publications
Read Publications using NBP1-85358.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24339976)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyclophilin B Antibody

  • Cyclophilin B
  • cyclophilin-like protein
  • CYPB
  • CYPBEC 5.2.1.8
  • CYP-S1
  • MGC2224
  • OI9MGC14109
  • peptidyl-prolyl cis-trans isomerase B
  • peptidylprolyl isomerase B (cyclophilin B)
  • PPIase B
  • PPIB
  • Rotamase B
  • S-cyclophilin
  • SCYLP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyclophilin B Antibody (NBP1-85358)(2)

Review for Cyclophilin B Antibody (NBP1-85358) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-85358:
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Simple Western
(1)
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Images Ratings Applications Species Date Details
Simple Western Cyclophilin B NBP1-85358
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4
reviewed by:
Verified Customer
Simple Western Mouse 04/21/2017
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Summary

ApplicationSimple Western
Sample Testedmouse liver lysate
SpeciesMouse
LotA106997

Comments

CommentsUsed NBP1-85358 anti-Cyclophilin B antibody with B6 Mouse Liver Lysate @ 1mg/ml because of the lack of sensitivity of another antibody. Runs as 28 kDa on the 12 to 230 kDa kit. Antibody titration of 1:6.25 to 1:200 is shown.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyclophilin B Antibody (NBP1-85358) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Cyclophilin B Antibody (NBP1-85358)

Discover related pathways, diseases and genes to Cyclophilin B Antibody (NBP1-85358). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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Verified Customer
04/21/2017
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol PPIB