Cyclophilin B Antibody - BSA Free

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.

Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody [NBP1-85358] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human thyroid gland shows high expression.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human prostate shows weak granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.1 mg/ml

Cyclophilin B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cyclophilin B Antibody - BSA Free (NBP1-85358) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-Cyclophilin B Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPIB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Simple Western 8 ug/mL
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
  See Simple Western Antibody Database for Simple Western validation: Tested in Titrated to saturation using various models, separated by Size, antibody dilution of 8 ug/mL
• Control Peptide:

  Cyclophilin B Protein (NBP1-85358PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Affinity purified

Alternate Names for Cyclophilin B Antibody - BSA Free

• Cyclophilin B
• cyclophilin-like protein
• CYPB
• CYPBEC 5.2.1.8
• CYP-S1
• MGC2224
• OI9MGC14109
• peptidyl-prolyl cis-trans isomerase B
• peptidylprolyl isomerase B (cyclophilin B)
• PPIase B
• PPIB
• Rotamase B
• S-cyclophilin
• SCYLP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclophilin B Antibody (NBP1-85358)(2)

Publications using NBP1-85358

• Hayashi T, Saito T, Fujimura T et al. Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma. PLoS One 2013-01-01 [PMID: 24339976]
• van Dijk FS, Nesbitt IM, Zwikstra EH et al. PPIB mutations cause severe osteogenesis imperfecta. Am J Hum Genet 2009-10-01 [PMID: 19781681]

Review for Cyclophilin B Antibody (NBP1-85358) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Rob Wilpan Simple Western Mouse 04/21/2017

Summary

• Application: Simple Western
• Sample Tested: mouse liver lysate
• Species: Mouse
• Lot: A106997

Comments

• Comments: Used NBP1-85358 anti-Cyclophilin B antibody with B6 Mouse Liver Lysate @ 1mg/ml because of the lack of sensitivity of another antibody. Runs as 28 kDa on the 12 to 230 kDa kit. Antibody titration of 1:6.25 to 1:200 is shown.

Bioinformatics

• Gene Symbol: PPIB