Presenilin-1 Antibody (APS 11)

Images

 
Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).
Immunocytochemistry/ Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.
Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen ...read more
Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
APS 11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Presenilin-1 Antibody (APS 11) Summary

Immunogen
Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.
Localization
Integral membrane protein. Golgi and endoplasmic reticulum. Bound to NOTCH1 also at the cell surface.
Specificity
No cross-reactivity is seen with presenilin 2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSEN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • ELISA 4.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.75 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 0.75 ug/ml
  • Immunoprecipitation 1:10 - 1:500
Application Notes
IHC-P: Staining of both the human brain plaque core and dystrophic neurites. WB: Detects an approx. 28 kDa protein representing PS1 N-terminus cleavage product.
Control
Brain Lysate (NB820-59177)
Presenilin-1 Lysate (NBP2-65407)
Reviewed Applications
Read 1 Review rated 4
using
NB300-749 in the following applications:

Publications
Read Publication using
NB300-749 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Presenilin-1 Antibody (APS 11)

  • AD3EC 3.4.23.-
  • Alzheimer disease 3
  • EC 3.4.23
  • FAD
  • presenilin 1
  • Presenilin1
  • Presenilin-1
  • Protein S182
  • PS-1
  • PS1presenilin-1
  • PSEN1
  • PSNL1
  • S182

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2). Presenilins form the catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Evidence suggests cleavage of APP by gamma-secretase and the production of Abeta (42 aa) is likely to play a important or casual role in the development of Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for Presenilin-1 Antibody (NB300-749)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Presenilin-1 Antibody (NB300-749) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using NB300-749:
Filter by Applications
WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Presenilin-1 NB300-749
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reviewed by:
Anonymous
WB 12/07/2012
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Summary

ApplicationWestern Blot
Pub Med ID22764098
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Presenilin-1 Antibody (NB300-749). (Showing 1 - 1 of 1 FAQ).

  1. What is the exact immuogen?
    • The immunogen range for this antibody falls between amino acids 340-370.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Presenilin-1 Antibody (NB300-749)

Discover related pathways, diseases and genes to Presenilin-1 Antibody (NB300-749). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Presenilin-1.

Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.

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Recent Reviews

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Anonymous
12/07/2012
Application: WB
Species:

Bioinformatics

Gene Symbol PSEN1
Entrez
Uniprot