Pref-1/DLK1/FA1 Antibody (3A10)

Western Blot: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)

Immunocytochemistry/Immunofluorescence: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Immunofluorescence analysis of U251 cells using DLK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Flow cytometric analysis of NIH/3T3 cells using DLK1 mouse mAb (green) and negative control (red).

ELISA: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Pref-1/DLK1/FA1 Antibody (3A10) Summary

• Immunogen: Purified recombinant fragment of human Pref-1/DLK1/FA1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLK1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Pref-1/DLK1/FA1 Antibody (3A10)

• delta-like 1 homolog (Drosophila)
• DLK
• DLK1
• DLK-1
• FA1
• FA1fetal antigen 1
• pG2
• pG2delta-like homolog (Drosophila)
• preadipocyte factor 1
• Pref1
• Pref-1
• protein delta homolog 1
• secredeltin
• ZOG

Background

DLK1 may have a role in neuroendocrine differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLK1