MAP3K12 Antibody

Western Blot: MAP3K12 Antibody [NBP2-17218] - A. 20 ug mouse embryonic stem cell whole cell lysate/extract 7.5 % SDS-PAGEDLK/MAP3K12 antibody dilution: 1:1000

Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218] - Frozen sectioned E13.5 Rat brain. Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: MAP3K12 Antibody [NBP2-17218] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000.

Western Blot: MAP3K12 Antibody [NBP2-17218] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody iluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP2-17218] - Analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Western Blot: MAP3K12 Antibody [NBP2-17218] -Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody diluted at 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MAP3K12 Antibody Summary

• Description: Novus Biologicals Rabbit MAP3K12 Antibody (NBP2-17218) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-MAP3K12 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of mouse MAP3K12. The exact sequence is proprietary.
• Localization: Cytoplasm; Membrane; Peripheral membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K12
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western
  • Western Blot 1:500-1:3000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Retinal Ganglion Cells, separated by Size
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MAP3K12 Antibody

• DLKEC 2.7.11.25
• Dual leucine zipper bearing kinase
• EC 2.7.11
• Leucine-zipper protein kinase
• MAPK-upstream kinase
• MEKK12
• mitogen-activated protein kinase kinase kinase 12
• Mixed lineage kinase
• MUKdual leucine zipper kinase DLK
• protein kinase MUK
• ZPKleucine zipper protein kinase
• ZPKP1

Background

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MAP3K12 Antibody (NBP2-17218)(2)

Publications using NBP2-17218

• Chandrasekhar A, Komirishetty P, Areti A et al. Dual Specificity Phosphatases Support Axon Plasticity and Viability Mol. Neurobiol. 2020-09-21 [PMID: 32959171]
• Welsbie DS, Mitchell KL, Jaskula-Ranga V et al. Enhanced Functional Genomic Screening Identifies Novel Mediators of Dual Leucine Zipper Kinase-Dependent Injury Signaling in Neurons. Neuron. 2017-06-21 [PMID: 28641113]

Bioinformatics

• Gene Symbol: MAP3K12
• Uniprot:
  • Q60700(Mouse)