PRDM4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PRDM4 Antibody [NBP1-80921] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunohistochemistry: PRDM4 Antibody [NBP1-80921] - Staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRDM4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LSTSHNLASLESVSLHEVGLSLEPVAVSSITQEVAMGTGHVDVSSDSLSFVSPSLQMEDSNSNKENMATLFTIWCTLCDRAYPSDCPEHGPVTFVPDTPIESRARLSLPKQLVLRQSIVGAEVGVWTGETIPVRTCFGPLIG
• Predicted Species: Mouse (96%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRDM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PRDM4 Protein (NBP1-80921PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23918801)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PRDM4 Antibody - BSA Free

• MGC45046
• PFM1PR domain-containing protein 4
• PR domain containing 4
• PR domain zinc finger protein 4
• PR-domain zinc-finger protein PFM1

Background

PRDM4/PFM1 may function as a transcription factor involved in cell differentiation. It contains 6 C2H2-type zinc fingers and is expressed in many tissues. Highly expressed in ovary, testis, pancreas, brain, heart and prostate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRDM4 Antibody (NBP1-80921)(1)

Publication using NBP1-80921

• Bogani D, Morgan MA, Nelson AC et al. The PR/SET Domain Zinc Finger Protein Prdm4 Regulates Gene Expression in Embryonic Stem Cells but Plays a Nonessential Role in the Developing Mouse Embryo. Mol Cell Biol 2013-10-01 [PMID: 23918801]

Bioinformatics

• Gene Symbol: PRDM4
• Entrez:
  • 11108(Human)
• Uniprot:
  • Q9UKN5(Human)