PRDM5 Antibody (7D4C12) - BSA Free

Western Blot: PRDM5 Antibody (7D4C12) [NBP2-52540] - Western blot analysis using PRDM5 mouse mAb against HL-60 (1), NIH/3T3 (2), SW480 (3), and HEK293 (4) cell lysate.

Immunocytochemistry/Immunofluorescence: PRDM5 Antibody (7D4C12) [NBP2-52540] - Analysis of MCF-7 cells using PRDM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow Cytometry: PRDM5 Antibody (7D4C12) [NBP2-52540] - Analysis of HeLa cells using PRDM5 mouse mAb (green) and negative control (red).

Western Blot: PRDM5 Antibody (7D4C12) [NBP2-52540] - Analysis using PRDM5 mAb against human PRDM5 (AA: 1-100) recombinant protein. (Expected MW is 37.6 kDa)

Western Blot: PRDM5 Antibody (7D4C12) [NBP2-52540] - Analysis using PRDM5 mAb against HEK293 (1) and PRDM5 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: PRDM5 Antibody (7D4C12) [NBP2-52540] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 7D4C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PRDM5 Antibody (7D4C12) - BSA Free Summary

• Description: Novus Biologicals Mouse PRDM5 Antibody (7D4C12) - BSA Free (NBP2-52540) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-PRDM5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDM5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31119897).
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PRDM5 Antibody (7D4C12) - BSA Free

• PFM2PR domain-containing protein 5
• PR domain containing 5
• PR domain zinc finger protein 5

Background

Official Gene Symbol PRDM5 Gene ID 11107(Human) Gene Map Locus 4q25-q26(Human) PRDM5, a novel member of PR domain protein family, contains a PR-domain at the NH2 terminus followed by 16 zinc finger motifs. It is found to have a role in cell differentiation and tumorigenesis. Reports suggest PRDM5 causes G2/M arrest and apoptosis upon infection of tumor cells and has been found to be silenced in breast, ovarian and lung cancers, suggesting a potential clinical significance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRDM5 Antibody (NBP2-52540)(2)

Publications using NBP2-52540

• Teng JJ, Zhao WJ, Zhang XL Et al. Downregulation of promoter methylation gene PRDM5 contributes to the development of tumor proliferation and predicts poor prognosis in gastric cancer Journal of Cancer 2021-10-03 [PMID: 34659579]
• Zhou P, Chen X, Li M Overexpression of PRDM5 promotes acute myeloid leukemia cell proliferation and migration by activating the JNK pathway Cancer Med 2019-05-23 [PMID: 31119897] (IHC-P, Human)

Bioinformatics

• Gene Symbol: PRDM5