PRDM16/MEL1 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

PRDM16/MEL1 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant mouse PRDM16
  Lys537-Glu688
  Accession # A2A935.1
• Specificity: Detects mouse and human PRDM16 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDM16/MEL1 Antibody [Alexa Fluor® 750]

• MDS1/EVI1-like gene 1
• MEL1
• MEL1KIAA1675PFM13MGC166915
• PFM13
• PR domain containing 16
• PR domain zinc finger protein 16
• PR domain-containing protein 16
• PRDM16
• Transcription factor MEL1

Background

PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.