PRDC/GREM2 Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

PRDC/GREM2 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse PRDC
  Arg22-Gln168
  Accession # O88273
• Specificity: Detects mouse PRDC in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse (rm) Cerberus, rmGremlin, rmDAN, recombinant human (rh) COCO, rhGremlin, and rmBMP-4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDC/GREM2 Antibody [Alexa Fluor® 594]

• CKTSF1B2
• CKTSF1B2FLJ21195
• Cysteine knot superfamily 1, BMP antagonist 2
• DAN domain family member 3
• DAND3
• DAND3Prdc
• GREM2
• gremlin 2
• gremlin 2, cysteine knot superfamily, homolog
• gremlin-2
• PRDC
• PRDCgremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
• Protein related to DAN and cerberus

Background

PRDC (protein related to DAN and Cerberus) is a secreted cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, Dan, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot, which form an additional intrasubunit disulfide bond (1, 2). Some members of this family, including PRDC, have an additional cysteine residue used for dimerization (1, 2). Of all the CAN family members, PRDC is most closely related to Gremlin, displaying 52% amino acid sequence identity. PRDC was first identified in a screen for developmentally regulated genes by gene trapping in embryonic stem cells (3). PRDC expression is detected by in situ hybridization in the dorsal edge of the spinal cord at E10.5, in commissural neurons in the caudal part of the spinal cord two days later (3), and in the granulosa cells of selective ovarian follicles (4). In the adult, abundant levels of PRDC are detected by RT-PCR in the mouse ovary, brain, and spleen, and to a lesser degree in the colon, kidney, lung, liver, and uterus (4). PRDC acts as a specific BMP antagonist, binding to and blocking signaling induced by BMP-2 or -4, but not Activin or TGF-beta (4). Thus, PRDC expression in the ovary could be involved in follicular development by antagonizing the inhibitory effects of BMPs on FSH stimulation of progesterone (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.