PRDC/GREM2 Antibody

Images

 
There are currently no images for PRDC/GREM2 Antibody (AF2069).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

PRDC/GREM2 Antibody Summary

Immunogen
E. coli-derived recombinant mouse PRDC
Arg22-Gln168
Accession # O88273
Specificity
Detects mouse PRDC in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse (rm) Cerberus, rmGremlin, rmDAN, recombinant human (rh) COCO, rhGremlin, and rmBMP-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GREM2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse PRDC/GREM2 Antibody (Catalog # AF2069)
ELISA Detection: Mouse PRDC/GREM2 Biotinylated Antibody (Catalog # BAF2069)
Standard: Recombinant Mouse PRDC/GREM2 (Catalog # 2069-PR)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDC/GREM2 Antibody

  • CKTSF1B2
  • CKTSF1B2FLJ21195
  • Cysteine knot superfamily 1, BMP antagonist 2
  • DAN domain family member 3
  • DAND3
  • DAND3Prdc
  • GREM2
  • gremlin 2
  • gremlin 2, cysteine knot superfamily, homolog
  • gremlin-2
  • PRDC
  • PRDCgremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
  • Protein related to DAN and cerberus

Background

PRDC (protein related to DAN and Cerberus) is a secreted cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, Dan, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot, which form an additional intrasubunit disulfide bond (1, 2). Some members of this family, including PRDC, have an additional cysteine residue used for dimerization (1, 2). Of all the CAN family members, PRDC is most closely related to Gremlin, displaying 52% amino acid sequence identity. PRDC was first identified in a screen for developmentally regulated genes by gene trapping in embryonic stem cells (3). PRDC expression is detected by in situ hybridization in the dorsal edge of the spinal cord at E10.5, in commissural neurons in the caudal part of the spinal cord two days later (3), and in the granulosa cells of selective ovarian follicles (4). In the adult, abundant levels of PRDC are detected by RT-PCR in the mouse ovary, brain, and spleen, and to a lesser degree in the colon, kidney, lung, liver, and uterus (4). PRDC acts as a specific BMP antagonist, binding to and blocking signaling induced by BMP-2 or -4, but not Activin or TGF-beta (4). Thus, PRDC expression in the ovary could be involved in follicular development by antagonizing the inhibitory effects of BMPs on FSH stimulation of progesterone (4).

  1. Pearce, J. et al. (1999) Dev. Biol. 209:99.
  2. Avsian-Kretchmer, O. and A. Hsueh (2004) Molecular Endocrinology 18:1.
  3. Minabe-Saegusa, C. et al. (1998) Develop. Growth Differ. 40:343.
  4. Sudo, S. et al. (2004) Jour. Biol. Chem. 279:23134.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

5190-GR
Species: Hu
Applications: BA
AF755
Species: Mu
Applications: Block, WB
NBP2-01223
Species: Hu
Applications: Flow, IHC, IHC-P, WB
AF1075
Species: Hu
Applications: WB
314-BP/CF
Species: Hu
Applications: BA, BA
NBP2-46321
Species: Hu, Mu, Rt
Applications: IHC, WB
355-BM/CF
Species: Hu, Mu, Rt
Applications: BA
6057-NG/CF
Species: Hu
Applications: BA
NBP2-24677
Species: Ca, Eq, Hu, Pm
Applications: ICC/IF, IHC, IHC-P, WB
AF3014
Species: Hu
Applications: Block, IHC, WB
H00001523-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
853-G5/CF
Species: Mu
Applications: BA
QET00B
Species: Hu
Applications: ELISA
AF7034
Species: Hu
Applications: WB
354-BP/CF
Species: Hu
Applications: BA
739-G9/CF
Species: Mu
Applications: BA
758-CN/CF
Species: Mu
Applications: BA
3218-ND/CF
Species: Hu
Applications: BA
AF2069
Species: Mu
Applications: WB, ELISA(Cap)

Publications for PRDC/GREM2 Antibody (AF2069) (0)

There are no publications for PRDC/GREM2 Antibody (AF2069).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PRDC/GREM2 Antibody (AF2069) (0)

There are no reviews for PRDC/GREM2 Antibody (AF2069). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PRDC/GREM2 Antibody (AF2069) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PRDC/GREM2 Products

Bioinformatics Tool for PRDC/GREM2 Antibody (AF2069)

Discover related pathways, diseases and genes to PRDC/GREM2 Antibody (AF2069). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PRDC/GREM2 Antibody (AF2069)

Discover more about diseases related to PRDC/GREM2 Antibody (AF2069).
 

Pathways for PRDC/GREM2 Antibody (AF2069)

View related products by pathway.

PTMs for PRDC/GREM2 Antibody (AF2069)

Learn more about PTMs related to PRDC/GREM2 Antibody (AF2069).

Blogs on PRDC/GREM2

There are no specific blogs for PRDC/GREM2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PRDC/GREM2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GREM2
Uniprot