PRAT4B/CNPY4 Antibody

Images

 
Western blot shows lysates of rat lung tissue and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015) followed by ...read more
RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

PRAT4B/CNPY4 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derive recombinant mouse PRAT4B
Thr30-Leu245
Accession # Q8BQ47
Specificity
Detects mouse and rat PRAT4B in Western blots.  In direct ELISAs, less than 5% cross-reactivity with recombinant human PRAT4B and recombinant mouse PRAT4A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CNPY4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF5015 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRAT4B/CNPY4 Antibody

  • canopy 4 homolog (zebrafish)
  • CNPY4
  • MGC40499PRAT4BPRotein Associated with Tlr4
  • PRAT4B
  • protein canopy homolog 4

Background

PRAT4B (protein associated with TLR4-B; also Cnpy4, protein canopy homolog 4) is a member of the canopy family of proteins. It is expressed by diverse cell types, including macrophages, fibroblasts and B cells. PRAT4B appears to be both secreted, and retained internally, where it may stabilize immature TLR protein expression in the ER, and impact its appearance on the cell surface. Mouse PRAT4B is 245 amino acids (aa) in length. It contains a 27 aa signal sequence plus one coiled-coil region (aa 209 - 237). There is one potential splice variant that shows a 13 aa substitution for the C-terminal nine amino acids. Over aa 30 - 245, mouse PRAT4B is 95% and 87% aa identical to rat and human PRAT4B, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRAT4B/CNPY4 Antibody (AF5015)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol CNPY4
Uniprot