PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric)

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PPAR gamma/NR1C3 Assay Kit (Colorimetric) [KA1358] - Standard Curve Example

Product Details

Summary
Applications Func
Suitable Sample Type
Nuclear Extracts

Order Details

PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric) Summary

Description
PPAR gamma/NR1C3 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting PPAR gamma/NR1C3 transcription factor DNA binding activity in nuclear extracts.
Assay Type
Colorimetric
Sample Volume
10 uL/well. All samples should be assayed in duplicate at minimum.
Kit Type
Transcription Factor Assay Kit (Colorimetric)
Gene
PPARG

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 96-Well Cover Sheet
  2. Polysorbate 20
  3. Transcription Factor Antibody Binding Buffer (10x)
  4. Transcription Factor Binding Assay Buffer (4x)
  5. Transcription Factor Developing Solution
  6. Transcription Factor Goat Anti-Rabbit HRP Conjugate
  7. Transcription Factor PPAR 96-Well Strip Plate
  8. Transcription Factor PPAR Competitor dsDNA
  9. Transcription Factor PPAR(gamma) Positive Control
  10. Transcription Factor PPAR(gamma) Primary Antibody
  11. Transcription Factor Reagent A
  12. Transcription Factor Stop Solution
  13. Wash Buffer Concentrate (400x)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric)

  • CIMT1
  • NR1C3
  • NR1C3GLM1
  • Nuclear receptor subfamily 1 group C member 3
  • peroxisome proliferator-activated receptor gamma 1
  • peroxisome proliferator-activated receptor gamma
  • PPAR gamma
  • PPARG
  • PPARG1peroxisome proliferative activated receptor, gamma
  • PPARG2PPARgamma
  • PPAR-gamma

Background

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors. PPAR gamma is the most widely studied PPAR and exists in two protein isoforms (Gamma 1 and Gamma 2) due to use of an alternative promoter and alternative splicing. PPAR gamma is primarily expressed in adipose tissue and to a lesser extent in the colon, immune system, and the retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

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Bioinformatics

Gene Symbol PPARG