PPAR delta/NR1C2 Antibody

Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - Staining of MOLT4 lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - PPAR delta in HeLa cell lysate (25 ug) using anti-PPAR delta antibody. Image from verified customer review.

• Reactivity: Hu, Rt, Mu, Po, Bv
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PPAR delta/NR1C2 Antibody Summary

• Description: Novus Biologicals Goat PPAR delta/NR1C2 Antibody (NB300-962) is a polyclonal antibody validated for use in WB, ELISA and IP. Anti-PPAR delta/NR1C2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CHPLLQEIYKDMY corresponding to C-Terminus according to NP_006229.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPARD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 55 kDa band observed in human Lymphoblastoid and T-cell line MOLT4 lysates (calculated MW of 49.9 kDa band according to NP_006229.1). Use in immunoprecipitation reported in scientific literature (PMID: 23555761).
• Control:

  PPAR delta/NR1C2 Overexpression Lysate

• Control Peptide:

  PPAR delta/NR1C2 Peptide (NB300-962PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23555761).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPAR delta/NR1C2 Antibody

• FAAR
• MGC3931
• NR1C2
• NR1C2PPAR-delta
• NUC1
• NUC1PPAR-beta
• NUCI
• NUCII
• Nuclear hormone receptor 1
• Nuclear receptor subfamily 1 group C member 2
• peroxisome proliferative activated receptor, delta
• Peroxisome proliferator-activated receptor beta
• peroxisome proliferator-activated receptor delta
• PPAR delta
• PPARB
• PPARD

Background

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPAR delta/NR1C2 Antibody (NB300-962)(2)

Publications using NB300-962

• Castillero E, Alamdari N, Aversa Z et al. PPARbeta/delta Regulates Glucocorticoid- and Sepsis-Induced FOXO1 Activation and Muscle Wasting PLoS One 2013-01-01 [PMID: 23555761] (IP, WB, Rat)
• Park BH, Vogelstein B, Kinzler KW. Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells. Proc Natl Acad Sci U S A 2001-02-27 [PMID: 11226285]

Bioinformatics

• Gene Symbol: PPARD
• Entrez:
  • 5467(Human)
  • 19015(Mouse)
  • 25682(Rat)
• Uniprot:
  • Q03181(Human)