PPAR delta/NR1C2 Antibody

Images

 
Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - NB300-962 (0.3 ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - PPAR delta in HeLa cell lysate (25ug) using anti-PPAR delta antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PPAR delta/NR1C2 Antibody Summary

Immunogen
Peptide with sequence CHPLLQEIYKDMY corresponding to C-Terminus according to NP_006229.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 55 kDa band observed in human Lymphoblastoid and T-cell line MOLT4 lysates (calculated MW of 49.9 kDa band according to NP_006229.1).
Positive Control
PPAR delta/NR1C2 Lysate (NBL1-14632)
Publications
Read Publications using
NB300-962 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPAR delta/NR1C2 Antibody

  • FAAR
  • MGC3931
  • NR1C2
  • NR1C2PPAR-delta
  • NUC1
  • NUC1PPAR-beta
  • NUCI
  • NUCII
  • Nuclear hormone receptor 1
  • Nuclear receptor subfamily 1 group C member 2
  • peroxisome proliferative activated receptor, delta
  • Peroxisome proliferator-activated receptor beta
  • peroxisome proliferator-activated receptor delta
  • PPAR delta
  • PPARB
  • PPARD

Background

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPAR delta/NR1C2 Antibody (NB300-962)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPARD
Entrez
Uniprot