PPAR alpha/NR1C1 Antibody

Product Discontinued

PPAR alpha/NR1C1 Antibody Summary

• Immunogen: Synthetic peptide from mouse PPARA / PPAR amino acids 1-18.
• Epitope: N-Terminus
• Specificity: PPAR alpha
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:8000-1:32000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Western Blot 1:500-1:2000

Reactivity Notes

Feline reactivity reported in scientific literature (PMID: 29920828).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 0.98 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPAR alpha/NR1C1 Antibody

• alpha
• hPPAR
• NR1C1
• NR1C1MGC2237
• Nuclear receptor subfamily 1 group C member 1
• peroxisome proliferator-activated receptor alpha
• PPAR alpha
• PPARA
• PPARalpha
• PPAR-alpha
• PPARMGC2452

Background

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPAR alpha/NR1C1 Antibody (NBP1-03288)(4)

Publications using NBP1-03288

• Pereira BMV, Thieme K, de Araujo L, Rodrigues AC Lack of adiponectin in mice accelerates high-fat diet-induced progression of chronic kidney disease Life Sci. 2020-07-08 [PMID: 32652137] (WB, Mouse)
• Zhong X, Yu J, Frazier K et al. Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m6A mRNA Methylation. Cell Rep. 2018-11-13 [PMID: 30428350] (WB, Human)
• Miragliotta V, Ricci PL, Albanese F et al. Cannabinoid receptor types 1 and 2 and peroxisome proliferator-activated receptor-alpha: distribution in the skin of clinically healthy cats and cats with hypersensitivity dermatitis Vet. Dermatol. 2018-06-19 [PMID: 29920828] (IHC-P, Feline)
• Baker BG, Ball GR, Rakha EA et al. Lack of expression of the proteins GMPR2 and PPARalpha are associated with the basal phenotype and patient outcome in breast cancer Breast Cancer Res Treat 2012-12-04 [PMID: 23208589] (IF/IHC, Human)

Reviews for PPAR alpha/NR1C1 Antibody (NBP1-03288) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Jason Johnson ICC Human 09/24/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: Human primary macrophages
• Species: Human
• Lot: 39436

reviewed by: Jason Johnson WB Human 09/24/2013

Summary

• Application: Western Blot
• Sample Tested: Human macrophage lysate
• Species: Human
• Lot: 39436

FAQs for PPAR alpha/NR1C1 Antibody (NBP1-03288). (Showing 1 - 1 of 1 FAQs).

1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
   • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.

Bioinformatics

• Gene Symbol: PPARA
• Entrez:
  • 5465(Human)
  • 19013(Mouse)
• Uniprot:
  • Q07869()