PPAR alpha/NR1C1 Antibody

Product Discontinued

PPAR alpha/NR1C1 Antibody Summary

• Immunogen: Recombinant (partial), N-terminal
• Specificity: PPAR
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPAR alpha/NR1C1 Antibody

• alpha
• hPPAR
• NR1C1
• NR1C1MGC2237
• Nuclear receptor subfamily 1 group C member 1
• peroxisome proliferator-activated receptor alpha
• PPAR alpha
• PPARA
• PPARalpha
• PPAR-alpha
• PPARMGC2452

Background

PPAR (Peroxisome proliferator activated receptor) belongs to the nuclear hormone receptor family, NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. PPAR subfamily consists of three structurally related members, PPARa, PPARb/d, and PPARg (52 kD, 50 kD, and 58 kD, respectively). PPARa expressed in skeletal muscle, liver, heart and kidney. PPARb/d expressed in many tissues but markedly in brain, adipose tissue and skin. PPARg expressed in adipose tissue, skeletal muscle, spleen, heart, liver, placenta, lung and ovary. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. PPARa controls expression of genes implicated in lipid metabolism. PPARb/d stimulates fatty acid oxidation predominantly acting in muscle. PPARg is a key regulator of glucose homeostasis and adipogenesis. PPARs also plays an important role in controlling immune and inflammatory responses. The poly6371 antibody recognizes human PPAR a, b/d, and g isoforms and has been shown to be useful for immunoflorence staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PPAR alpha/NR1C1 Antibody (NB100-78572). (Showing 1 - 1 of 1 FAQ).

1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
   • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.

Bioinformatics

• Gene Symbol: PPARA
• Entrez:
  • 5465(Human)