PP14/Glycodelin Antibody [Unconjugated]

Western blot shows lysates of human uterus tissue and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human PP14/Glycodelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF842) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PP14/Glycodelin at approximately 24-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PP14/Glycodelin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PP14/Glycodelin
  Met19-Phe180 (Glu95Gly)
  Accession # P09466
• Specificity: Detects human PP14/Glycodelin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAEP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP14/Glycodelin Antibody [Unconjugated]

• alpha uterine protein
• GdF
• GdS
• Glycodelin
• glycodelin-A
• glycodelin-F
• glycodelin-S
• MGC138509
• MGC142288
• PAEP
• PEP
• Placental protein 14
• PP14 protein (placental protein 14)
• PP14
• PP14GDPAEGGdA
• pregnancy-associated endometrial a
• Pregnancy-associated endometrial alpha-2 globulin
• pregnancy-associated endometrial alpha-2-globulin
• progestagen-associated endometrial protein (placental protein 14
• progestagen-associated endometrial proteinPEG
• Progesterone-associated endometrial protein

Background

Placenta Protein 14 (PP14, also known as Glycodelin-A) is a 28-kDa glycoprotein that is mainly expressed by late secretory-phase endometrium and first-trimester gestational endometrium. It is present at high levels in amniotic fluid, and in the serum of pregnant women. PP14 has been reported to have immuno- suppressive activity that may play a role in fetal survival during pregnancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP14/Glycodelin Antibody (AF842)(3)

Publications using AF842

• A Luddi, V Pavone, B Semplici, L Governini, M Criscuoli, E Paccagnini, M Gentile, G Morgante, V Leo, G Belmonte, N Zarovni, P Piomboni Organoids of Human Endometrium: A Powerful In Vitro Model for the Endometrium-Embryo Cross-Talk at the Implantation Site Cells, 2020-04-30;9(5):. 2020-04-30 [PMID: 32366044] (IHC, Western Blot, Human)
• S Sørensen, V Myrhøj, TH Nguyen, P Aaslo, YB Hansen Affinity purification of native glycodelin from amniotic fluid for biological investigations and development of a glycodelin ELISA for clinical studies Protein Expr. Purif, 2016-10-03;130(0):73-80. 2016-10-03 [PMID: 27713060] (Western Blot, Human)
• Lee CL, Chiu PC, Pang PC, Chu IK, Lee KF, Koistinen R, Koistinen H, Seppala M, Morris HR, Tissot B, Panico M, Dell A, Yeung WS Glycosylation failure extends to glycoproteins in gestational diabetes mellitus: evidence from reduced alpha2-6 sialylation and impaired immunomodulatory activities of pregnancy-related glycodelin-A. Diabetes, 2011-02-07;60(3):909-17. 2011-02-07 [PMID: 21300843] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: PAEP
• Uniprot:
  • P09466()