POU6F2 Antibody

Western Blot: POU6F2 Antibody [NBP1-52038] - NBP1-52038 (0.3ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

POU6F2 Antibody Summary

• Immunogen: Peptide with sequence RSEMNAELRGEDK-C corresponding to N-Terminus according to NP_009183.3, NP_001159490.1.
• Epitope: RSEMNAELRGEDK-C
• Specificity: This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POU6F2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in mouse eye lysates (calculated MW of 73.3 kDa band according to human NP_009183.3 and mouse NP_778171.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POU6F2 Antibody

• POU class 6 homeobox 2
• Retina-derived POU domain factor 1
• retina-derived POU-domain factor-1
• RPF1
• RPF-1
• RPF-1class 6, transcription factor 2
• Wilms tumor suppressor locus
• WT5
• WTSL

Background

POU6F2 is a member of a gene family characterized by the presence of a bipartite DNA-binding domain, consisting of a POU-specific domain and a POU heterodomain, separated by a variable polylinker. POU domain family members are transcriptional regulators, many of which show highly restricted patterns of expression and are known to control cell type-specific differentiation pathways (see review by Phillips and Luisi, 2000 [PubMed 11183772]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POU6F2 Antibody (NBP1-52038)(1)

Publication using NBP1-52038

• Perotti D, De Vecchi G, Testi MA et al. Germline mutations of the POU6F2 gene in Wilms tumors with loss of heterozygosity on chromosome 7p14. Hum Mutat 2004-11-01 [PMID: 15459955]

Bioinformatics

• Gene Symbol: POU6F2
• Entrez:
  • 11281(Human)
• Uniprot:
  • NP_009183.3(Human)