POU6F2 Antibody

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Western Blot: POU6F2 Antibody [NBP1-52038] - NBP1-52038 (0.3ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

POU6F2 Antibody Summary

Immunogen
Peptide with sequence RSEMNAELRGEDK-C corresponding to N-Terminus according to NP_009183.3, NP_001159490.1.
Epitope
RSEMNAELRGEDK-C
Specificity
This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POU6F2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 65 kDa band observed in mouse eye lysates (calculated MW of 73.3 kDa band according to human NP_009183.3 and mouse NP_778171.2).
Publications
Read Publication using NBP1-52038.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POU6F2 Antibody

  • POU class 6 homeobox 2
  • Retina-derived POU domain factor 1
  • retina-derived POU-domain factor-1
  • RPF1
  • RPF-1
  • RPF-1class 6, transcription factor 2
  • Wilms tumor suppressor locus
  • WT5
  • WTSL

Background

POU6F2 is a member of a gene family characterized by the presence of a bipartite DNA-binding domain, consisting of a POU-specific domain and a POU heterodomain, separated by a variable polylinker. POU domain family members are transcriptional regulators, many of which show highly restricted patterns of expression and are known to control cell type-specific differentiation pathways (see review by Phillips and Luisi, 2000 [PubMed 11183772]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POU6F2 Antibody (NBP1-52038)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POU6F2
Entrez
Uniprot