ATOH7 Antibody

ATOH7 antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ATOH7 antibody (NBP3-48614) dilution: 1:2500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ATOH7 antibody (NBP3-48614) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ATOH7 antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ATOH7 antibody (NBP3-48614) dilution: 1:2500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

ATOH7 antibody detects ATOH7 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ATOH7 antibody (NBP3-48614) dilution: 1:1000. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ATOH7 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATOH7. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATOH7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:2500
  • Western Blot 1:5000-1:20000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ATOH7 Antibody

• ATH5
• atonal homolog 7 (Drosophila)
• BHLHA13
• bHLHa13protein atonal homolog 7
• Class A basic helix-loop-helix protein 13
• hATH5
• Helix-loop-helix protein hATH-5
• Math5

Background

ATOH7 is a member of the family of basic helix-loop-helix (bHLH) proteins with similarity to Drosophila'atonal,' a proneural bHLH gene that controls photoreceptor development (Brown et al., 2002 (PubMed11889557)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATOH7