POT1 Antibody

Western Blot: POT1 Antibody [NB100-56429] - Analysis of POT1 using POT1 polyclonal antibody Human HL60 cell lysate probed using POT1 antibody at 2 ug/ml.

Product Discontinued

POT1 Antibody Summary

• Immunogen: A portion of amino acids 450-500 of human POT1 was used as the immunogen.
• Specificity: The amino acid sequence used is 100% homologous in human, chimp and monkey, and 86% in bovine and dog. This antibody will detect variant 1 (634 amino acids) and variant 3 (518 amino acids) of POT1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Gel Super Shift Assays 1:1-1:100
  • Western Blot 1-3 ug/ml
• Application Notes: Gel Super Shift Assays: See Choi et al (2011).
• Control:

  POT1 Overexpression Lysate

Reactivity Notes

Predicted to react with Bovine, Chimpanzee, Canine and New World Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for POT1 Antibody

• DKFZp586D211
• HPOT1
• POT1 protection of telomeres 1 homolog
• POT1
• POT1-like telomere end-binding protein
• protection of telomeres 1 homolog (S. pombe)
• protection of telomeres protein 1

Background

POT1 is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Pot1 also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Its activity is TERT dependent but it does not increase TERT activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POT1 Antibody (NB100-56429)(3)

Publications using NB100-56429

• Choi KH, Farrell AS, Lakamp AS et al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res. 2011-11-01 [PMID: 21852327] (GS)
  
  Details:
  Tin2 (IMG-282) & POT1 (IMG-5343A). EMSA: HeLa cell line transfected with or without Flag-Tin2, Fig 4C.
• Kamranvar SA, Masucci MG. The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress. Leukemia. 2011-06-01 [PMID: 21394098] (WB)
  
  Details:
  WB: TRF2 (IMG-124), Tankyrase (IMG-146), POT1 (IMG-5343A), and Tin2 (IMG-282). EBNA1 positive or negative Ramos and BJAB cell lines, Fig 4A.
• Sowd G, Lei M, Opresko PL. Mechanism and substrate specificity of telomeric protein POT1 stimulation of the Werner syndrome helicase. Nucleic Acids Res. 2008-08-01 [PMID: 18583366] (WB, Human)
  
  Details:
  WB (Human Werner syndrome cells), Figs. 2A,C; 3A,B; 5A; 6.

Bioinformatics

• Gene Symbol: POT1
• Entrez:
  • 25913(Human)
• Uniprot:
  • AAW22613.1(Human)