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POT1 Antibody (M1-P1H5) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
M1-P1H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50255AF350
Alexa Fluor 405
NBP2-50255AF405
Alexa Fluor 488
NBP2-50255AF488
Alexa Fluor 532
NBP2-50255AF532
Alexa Fluor 594
NBP2-50255AF594
Alexa Fluor 647
NBP2-50255AF647
Alexa Fluor 700
NBP2-50255AF700
Alexa Fluor 750
NBP2-50255AF750
DyLight 350
NBP2-50255UV
DyLight 405
NBP2-50255V
DyLight 488
NBP2-50255G
DyLight 550
NBP2-50255R
DyLight 594
NBP2-50255DL594
DyLight 650
NBP2-50255C
DyLight 680
NBP2-50255FR
DyLight 755
NBP2-50255IR
FITC
NBP2-50255F
HRP
NBP2-50255H
Janelia Fluor 549
NBP2-50255JF549
Janelia Fluor 646
NBP2-50255JF646
Unconjugated
NBP2-50255
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POT1 Antibody (M1-P1H5) [Biotin] Summary

Immunogen
Peptide sequence - IPASEVLMDD
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POT1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

BLAST analysis of immunogen peptide has revealed 100% identity to mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Positive control(s): ELISA- Peptide immunogenWestern Blot- Hela cell extract (nuclear fraction)IF- Hela Cells. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for POT1 Antibody (M1-P1H5) [Biotin]

  • DKFZp586D211
  • HPOT1
  • POT1 protection of telomeres 1 homolog
  • POT1
  • POT1-like telomere end-binding protein
  • protection of telomeres 1 homolog (S. pombe)
  • protection of telomeres protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western: TRF-2 Antibody (4A794.15) [NB100-56506] - Image shows a specific band for TRF2 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Localization of candidate RNAs at telomeres. Localization of NEAT1 at telomeres in human U-2 OS cells. Cells were fixed and sequentially incubated with Abs against TRF-2 and AlexaFluor 488-conjugated anti-mouse IgG. Subsequently, the cells were hybridized with the RNA probes and subjected to fluorescence microscopy. Three different cells are shown. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0123387), licensed under a CC-BY licence.
Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis in human Jurkat cell lysate at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used.
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Species: Hu, Mu, Rt, Ma, Ma
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(1D8-1B6)
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Western Blot: ACD Antibody (1D8-1B6) [H00065057-M02] - Western Blot analysis of ACD expression in IMR-32 ( Cat # L008V1 ).
Immunocytochemistry/Immunofluorescence: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 2 ug/ml.
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(57-6)
NB110-68281
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.
Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.
(3 Publications)
Species: Hu, Mu, Rt, Pm
Applications: ICC/IF (-), WB, Simple Western, ELISA, Flow
TBP like protein
TLP Antibody
NBP1-31133
Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/Immunofluorescence: TBP like protein TLP Antibody [NBP1-31133] - NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLP protein stained by TLP antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP1-31133] - Paraffin-embedded mouse lung. TLP antibody dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Tripeptidyl-Peptidase
NBP1-31758
Western Blot: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Paraffin-embedded mouse liver. TPP1 antibody [N1C1] diluted at 1:500.
Species: Hu, Mu
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TIN2 Antibody
NBP2-55709
Western Blot: TIN2 Antibody [NBP2-55709] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunocytochemistry/Immunofluorescence: TIN2 Antibody [NBP2-55709] - Staining of human cell line U-2 OS shows localization to nuclear bodies.
Species: Hu
Applications: WB, ICC/IF
Alpha-TTP Antibody
NBP2-14870
Western Blot: Alpha-TTP Antibody [NBP2-14870] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with alpha TTP antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Alpha-TTP Antibody [NBP2-14870] - Analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Alpha-TTP Antibody [NBP2-14870] - Gastric cancer. alpha TTP antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Species: Hu, Mu, Rt, Ha
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TERF2IP Antibody
NB100-292
Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292] - Sections of human lung carcinoma.~~ Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
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TERT Antibody (2C4)
NB100-317
Western Blot: TERT Antibody (2C4) [NB100-317] - Increased levels of IFI16 protein in WI-38 cells reduce hTERT levels and inhibit telomerase activity. Total protein extracts prepared from WI-38 cells, either transfected with control (pCMV) vector (lane 1) or pCMV-IFI16 plasmid (lane 2), were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC-BY licence.
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Western Blot: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
Immunohistochemistry-Paraffin: MMAB Antibody (2G5) [NBP2-03417] Staining of paraffin-embedded Human lymph node tissue using anti-MMAB mouse monoclonal antibody.
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody, and then analyzed by flow cytometry.
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Applications: WB, Flow, IHC, IHC-P
ATR Antibody (2B5)
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Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
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Western Blot: ATM Antibody [NB100-104] - Detection of ATM in HeLa nuclear extract using ATM antibody [NB100-104]. Theoretical molecular weight 351 kDa.
Immunohistochemistry-Paraffin: ATM Antibody [NB100-104] - Staining of human tonsil, germinal center and mantle zone with ATM Antibody [NB100-104].
Immunocytochemistry/Immunofluorescence: ATM Antibody [NB100-104] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.05% Triton X-100. Then cells were incubated with ATM Antibody [NB100-104] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Western Blot: Tankyrase 1 Antibody [NB100-186] - Detection of tankyrase in DU145 whole cell lysates (50 ug) using NB 100-186. ECL detection - 30 seconds. Lane 1 - 1:500 primary dilution Lane 2 - 1:2,000 primary dilution
Immunocytochemistry/Immunofluorescence: Tankyrase 1 Antibody [NB100-186] - Tankyrase antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 hour at room temperature in 1XPBS. Image from verified customer review.
Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.
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POT1 Antibody
(M1-P1H5) [Biotin]
NBP2-50255B
POT1 Antibody (M1-P1H5) [Biotin]
Species: Hu
Applications: WB, ELISA, ICC/IF

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Product General Protocols

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WB Video Protocol
ICC/IF Video Protocol

FAQs for POT1 Antibody (NBP2-50255B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50255AF350
Alexa Fluor 405 NBP2-50255AF405
Alexa Fluor 488 NBP2-50255AF488
Alexa Fluor 532 NBP2-50255AF532
Alexa Fluor 594 NBP2-50255AF594
Alexa Fluor 647 NBP2-50255AF647
Alexa Fluor 700 NBP2-50255AF700
Alexa Fluor 750 NBP2-50255AF750
Biotin NBP2-50255B
DyLight 350 NBP2-50255UV
DyLight 405 NBP2-50255V
DyLight 488 NBP2-50255G
DyLight 550 NBP2-50255R
DyLight 594 NBP2-50255DL594
DyLight 650 NBP2-50255C
DyLight 680 NBP2-50255FR
DyLight 755 NBP2-50255IR
FITC NBP2-50255F
HRP NBP2-50255H
Janelia Fluor 549 NBP2-50255JF549
Janelia Fluor 646 NBP2-50255JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for POT1 Antibody (NBP2-50255B)

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Blogs on POT1.

Check out the latest blog posts on POT1.
Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.
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Bioinformatics

Gene Symbol POT1