PON1 Antibody - BSA Free

Western Blot: PON1 Antibody [NBP2-19893] - . Human tissue extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PON1 antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: PON1 Antibody [NBP2-19893] - Analysis of mouse liver. PON1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PON1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PON1 Antibody - BSA Free (NBP2-19893) is a polyclonal antibody validated for use in IHC and WB. Anti-PON1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human PON1 Recombinant protein.
• Localization: Secreted; extracellular space
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PON1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (81%), Pig (84%), Rabbit (85%), Bovine (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PON1 Antibody - BSA Free

• A-esterase 1
• Aromatic esterase 1
• arylesterase B-type
• EC 3.1.1.2
• EC 3.1.1.81
• EC 3.1.8.1
• ESA
• ESAesterase A
• K-45
• MVCD5
• paraoxonase 1
• PON 1
• PON1
• PONparaoxonase B-type
• serum aryldiakylphosphatase
• Serum aryldialkylphosphatase 1
• serum paraoxonase/arylesterase 1

Background

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PON1 Antibody (NBP2-19893)(2)

Publications using NBP2-19893

• S Lim, KR Dunlap, ME Rosa-Caldw, WS Haynie, LT Jansen, TA Washington, NP Greene Comparative plasma proteomics in muscle atrophy during cancer-cachexia and disuse: The search for atrokines Physiol Rep, 2020-10-01;8(19):e14608. 2020-10-01 [PMID: 33063952] (Immunohistochemistry, Mouse)
• Halim H, Pinkaew D, et al. Ticagrelor induces paraoxonase-1 (PON1) and better protects hypercholesterolemic mice against atherosclerosis compared to clopidogrel. PLoS One 2019-06-27 [PMID: 31242230] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: PON1
• Uniprot:
  • P27169(Human)