PON1 Antibody (4X7X2)

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of HepG2 cells, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187] - Immunohistochemistry of paraffin-embedded human liver using PON1 Rabbit mAb (NBP3-16187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Rat liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Mouse liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Human liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 4X7X2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PON1 Antibody (4X7X2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PON1 (P27169). TGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEE
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PON1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PON1 Antibody (4X7X2)

• A-esterase 1
• Aromatic esterase 1
• arylesterase B-type
• EC 3.1.1.2
• EC 3.1.1.81
• EC 3.1.8.1
• ESA
• ESAesterase A
• K-45
• MVCD5
• paraoxonase 1
• PON 1
• PON1
• PONparaoxonase B-type
• serum aryldiakylphosphatase
• Serum aryldialkylphosphatase 1
• serum paraoxonase/arylesterase 1

Background

Paraoxonase 1 (PON1) is an arylesterase capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. PON1 primarily hydrolyzes paroxon, an organophosphorus anticholinesterase compound produced in vivo by oxidation of the insecticide parathion, to produce p-nitrophenol. Polymorphisms in this gene are a risk factor in coronary artery disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PON1