Polypeptide GalNac Transferase 7/GALNT7 Antibody

Images

 
Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP1-32491] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP1-32491] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GALNT7 protein stained by GALNT7 ...read more
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP1-32491] - Paraffin-embedded Endometrial CA, using antibody at 1:100 dilution.
Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP1-32491] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Polypeptide GalNac Transferase 7/GALNT7 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GALNT7. The exact sequence is proprietary.
Localization
Golgi apparatus membrane, Single-pass type II membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GALNT7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (82%), Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Polypeptide GalNac Transferase 7/GALNT7 Antibody

  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.37
  • EC 2.4.1.41
  • GalNAcT7
  • GALNAC-T7
  • GALNT7
  • N-acetylgalactosaminyltransferase 7
  • Polypeptide GalNAc transferase 7
  • polypeptide N-acetylgalactosaminyltransferase 7
  • pp-GaNTase 7
  • Protein-UDP acetylgalactosaminyltransferase 7
  • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 7 (GalNAc-T7)

Background

This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GALNT7
Entrez