IL-10R alpha Antibody (37607)

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Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N
Clone
37607
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

IL-10R alpha Antibody (37607) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha
His22-Asn235
Accession # Q13651.1
Specificity
Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL‑10 R alpha.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL10RA
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.03-0.1 ug/mL
Publications
Read Publications using
MAB274 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10R alpha Antibody (37607)

  • CD210 antigen
  • CDw210a
  • HIL-10R
  • IBD28
  • IL-10 R alpha
  • IL-10 receptor subunit alpha
  • IL10R alpha
  • IL-10R subunit 1
  • IL-10R1
  • IL10RA
  • IL-10Ra
  • IL10RIL-10R subunit alpha
  • interleukin 10 receptor, alpha
  • interleukin-10 receptor alpha chain
  • Interleukin-10 receptor subunit 1
  • interleukin-10 receptor subunit alpha

Background

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10R alpha Antibody (MAB274)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Blocking, Flow Cytometry, Functional Assay, Neutralization.


Filter By Application
Blocking
(1)
Flow Cytometry
(2)
Functional Assay
(1)
Neutralization
(8)
All Applications
Filter By Species
Human
(12)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB274 Applications Species
R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019;10(1):5016. 2019 [PMID: 31676788] (Neutralization, Human) Neutralization Human
C Thepmalee, A Panya, M Junking, T Chieochans, PT Yenchitsom Inhibition of IL-10 and TGF-? receptors on dendritic cells enhances activation of effector T-cells to kill cholangiocarcinoma cells Hum Vaccin Immunother, 2018;0(0):0. 2018 [PMID: 29420117] (Neutralization, Human) Neutralization Human
Leonie Brockmann IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo J. Immunol, 2016;0(0):. 2016 [PMID: 28003377] (Functional Assay, Human) Functional Assay Human
Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages Sci Rep, 2016;6(0):31959. 2016 [PMID: 27558590] (Blocking, Human) Blocking Human
von Lanzenauer S, Wolk K, Hoflich C, Kunz S, Grunberg B, Docke W, Reineke U, Asadullah K, Sterry W, Volk H, Sabat R Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10&#039;s inhibitory mechanisms. Genes Immun, 2015;16(1):8-14. 2015 [PMID: 25472783] (Flow Cytometry, Human) Flow Cytometry Human
Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts. Eur J Immunol, 2014;44(6):1615-21. 2014 [PMID: 24643722] (Neutralization, Human) Neutralization Human
Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L TNF-alpha blockade induces IL-10 expression in human CD4+ T cells. Nat Commun, 2014;5(0):3199. 2014 [PMID: 24492460] (Neutralization, Human) Neutralization Human
Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo M, Arinaitwe E, Briggs J, Greenhouse B, Tappero J, Kamya M, Dorsey G, Feeney M IFNgamma/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children. PLoS Pathog, 2014;10(1):e1003864. 2014 [PMID: 24415936] (Neutralization, Human) Neutralization Human
Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection. J. Virol., 2008;82(7):3736-50. 2008 [PMID: 18216121] (Neutralization, Human) Neutralization Human
Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 2005;105(2):750-8. 2005 [PMID: 15374887] (Neutralization, Human) Neutralization Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol IL10RA
Uniprot