IL-10R alpha Antibody (37607)

Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 R alpha Monoclonal Antibody (Catalog # MAB274). The ND50 is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL).

• Reactivity: Hu
• Applications: B/N
• Clone: 37607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-10R alpha Antibody (37607) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha
  His22-Asn235
  Accession # Q13651.1
• Specificity: Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL‑10 R alpha .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL10RA
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.03-0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10R alpha Antibody (37607)

• CD210 antigen
• CDw210a
• HIL-10R
• IBD28
• IL-10 R alpha
• IL-10 receptor subunit alpha
• IL10R alpha
• IL-10R subunit 1
• IL-10R1
• IL10RA
• IL-10Ra
• IL10RIL-10R subunit alpha
• interleukin 10 receptor, alpha
• interleukin-10 receptor alpha chain
• Interleukin-10 receptor subunit 1
• interleukin-10 receptor subunit alpha

Background

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10R alpha Antibody (MAB274)(13)

Publications using MAB274

• S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022;13(1):3121. 2022 [PMID: 35660734] (Neutralization, Human)
• R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019;10(1):5016. 2019 [PMID: 31676788] (Neutralization, Human)
• C Thepmalee, A Panya, M Junking, T Chieochans, PT Yenchitsom Inhibition of IL-10 and TGF-? receptors on dendritic cells enhances activation of effector T-cells to kill cholangiocarcinoma cells Hum Vaccin Immunother, 2018;0(0):0. 2018 [PMID: 29420117] (Neutralization, Human)
• Leonie Brockmann IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo J. Immunol, 2016;0(0):. 2016 [PMID: 28003377] (Functional Assay, Human)
• Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages Sci Rep, 2016;6(0):31959. 2016 [PMID: 27558590] (Blocking, Human)
• von Lanzenauer S, Wolk K, Hoflich C, Kunz S, Grunberg B, Docke W, Reineke U, Asadullah K, Sterry W, Volk H, Sabat R Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10&#039;s inhibitory mechanisms. Genes Immun, 2015;16(1):8-14. 2015 [PMID: 25472783] (Flow Cytometry, Human)
• Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts. Eur J Immunol, 2014;44(6):1615-21. 2014 [PMID: 24643722] (Neutralization, Human)
• Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L TNF-alpha blockade induces IL-10 expression in human CD4+ T cells. Nat Commun, 2014;5(0):3199. 2014 [PMID: 24492460] (Neutralization, Human)
• Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo M, Arinaitwe E, Briggs J, Greenhouse B, Tappero J, Kamya M, Dorsey G, Feeney M IFNgamma/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children. PLoS Pathog, 2014;10(1):e1003864. 2014 [PMID: 24415936] (Neutralization, Human)
• Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection. J. Virol., 2008;82(7):3736-50. 2008 [PMID: 18216121] (Neutralization, Human)
• Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 2005;105(2):750-8. 2005 [PMID: 15374887] (Neutralization, Human)
• Chang WL, Baumgarth N, Yu D, Barry PA Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. J. Virol., 2004;78(16):8720-31. 2004 [PMID: 15280480] (Neutralization, Human)
• Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures. Blood, 2004;104(2):453-61. 2004 [PMID: 15031211] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL10RA
• Uniprot:
  • Q13651.1()