Polypeptide GalNac Transferase 4/GALNT4 Antibody

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Western Blot: GALNT4 Antibody [NBP1-69399] - This Anti-GALNT4 antibody was used in Western Blot of Fetal Brain tissue lysate at a concentration of 1ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Polypeptide GalNac Transferase 4/GALNT4 Antibody Summary

Immunogen
Synthetic peptides corresponding to GALNT4(UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)) The peptide sequence was selected from the C terminal of GALNT4. Peptide sequence ANLSLFGCHGQGGNQFFE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GALNT4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against GALNT4 and was validated on Western blot.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Polypeptide GalNac Transferase 4/GALNT4 Antibody

  • EC 2.4.1.41
  • FLJ93314
  • GALNACT4
  • GalNAc-T4
  • GALNT4
  • Polypeptide GalNAc transferase 4
  • polypeptide N-acetylgalactosaminyltransferase 4
  • pp-GaNTase 4
  • Protein-UDP acetylgalactosaminyltransferase 4
  • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 4 (GalNAc-T4)

Background

GALNT4 catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GALNT4
Entrez
Uniprot