POLR3F Antibody - BSA Free

Western Blot: POLR3F Antibody [NBP3-35233] - Western blot analysis of various lysates using POLR3F Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POLR3F Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POLR3F Antibody - BSA Free (NBP3-35233) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POLR3F (NP_006457.2).
  
  Sequence:
  MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR3F
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for POLR3F Antibody - BSA Free

• DNA-directed RNA polymerase III 39 kDa polypeptide
• DNA-directed RNA polymerase III subunit F
• DNA-directed RNA polymerase III subunit RPC6
• DNA-directed RNA polymerases III 39 kDa polypeptide
• polymerase (RNA) III (DNA directed) polypeptide F (39 kDa)
• polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
• RNA polymerase III 39 kDa subunit
• RNA polymerase III C39 subunit
• RNA polymerase III subunit C6
• RPC39MGC13517
• RPC6

Background

POLR3F is encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR3F