POLR3F Antibody

Images

 
Western Blot: POLR3F Antibody [NBP1-76929] - Human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: POLR3F Antibody [NBP1-76929] - Immunofluorescence of POLR3F in human brain tissue with POLR3F antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: POLR3F Antibody [NBP1-76929] - Human brain tissue with POLR3F antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

POLR3F Antibody Summary

Immunogen
Antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F. The immunogen is located within the first 50 amino acids of POLR3F.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR3F
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
POLR3F Peptide (NBP1-76929PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POLR3F Antibody

  • DNA-directed RNA polymerase III 39 kDa polypeptide
  • DNA-directed RNA polymerase III subunit F
  • DNA-directed RNA polymerase III subunit RPC6
  • DNA-directed RNA polymerases III 39 kDa polypeptide
  • polymerase (RNA) III (DNA directed) polypeptide F (39 kDa)
  • polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
  • RNA polymerase III 39 kDa subunit
  • RNA polymerase III C39 subunit
  • RNA polymerase III subunit C6
  • RPC39MGC13517
  • RPC6

Background

The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLR3F
Uniprot