POLR3F Antibody - BSA Free

Western Blot: POLR3F Antibody [NBP1-76929] - Human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry: POLR3F Antibody - BSA Free [NBP1-76929] - Immunohistochemistry of POLR3F in human brain tissue with POLR3F antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: POLR3F Antibody - BSA Free [NBP1-76929] - Immunofluorescence of POLR3F in human brain tissue with POLR3F antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

POLR3F Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POLR3F Antibody - BSA Free (NBP1-76929) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F. The immunogen is located within the first 50 amino acids of POLR3F. Amino Acid Squence: MAEVKVKVQPPDADPVEIEN
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR3F
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  POLR3F Peptide (NBP1-76929PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for POLR3F Antibody - BSA Free

• DNA-directed RNA polymerase III 39 kDa polypeptide
• DNA-directed RNA polymerase III subunit F
• DNA-directed RNA polymerase III subunit RPC6
• DNA-directed RNA polymerases III 39 kDa polypeptide
• polymerase (RNA) III (DNA directed) polypeptide F (39 kDa)
• polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
• RNA polymerase III 39 kDa subunit
• RNA polymerase III C39 subunit
• RNA polymerase III subunit C6
• RPC39MGC13517
• RPC6

Background

The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR3F
• Uniprot:
  • CAC11110(Human)