POLR2E Antibody - BSA Free

Images

 
Independent Antibodies: Western Blot: POLR2E Antibody [NBP2-49577] - Analysis using Anti-POLR2E antibody NBP2-49577 (A) shows similar pattern to independent antibody NBP2-57185 (B).
Immunohistochemistry-Paraffin: POLR2E Antibody [NBP2-49577] - Staining of human colon shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
 

Independent Antibodies

       

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Catalog# & Formulation Size Price

POLR2E Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit POLR2E Antibody - BSA Free (NBP2-49577) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQ
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
POLR2E Recombinant Protein Antigen (NBP2-49577PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for POLR2E Antibody - BSA Free

  • DNA directed RNA polymerase II 23 kda polypeptide
  • DNA-directed RNA polymerase II 23 kDa polypeptide
  • DNA-directed RNA polymerase II subunit E
  • DNA-directed RNA polymerases I, II, and III subunit RPABC1
  • hRPB25
  • hsRPB5
  • polymerase (RNA) II (DNA directed) polypeptide E (25kD)
  • polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
  • RPABC1
  • RPB5 homolog
  • RPB5
  • XAP4RNA polymerases I, II, and III subunit ABC1

Background

POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLR2E