POLR2E Antibodies
We offer POLR2E Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each POLR2E Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our POLR2E Antibodies can be used in a variety of model species: Canine, Human, Mouse, Primate, Rat. Use the list below to choose the POLR2E Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our POLR2E polyclonal antibodies and browse our POLR2E monoclonal antibody catalog.
Alternate Names for POLR2E Antibodies
anti-POLR2E antibody, anti-DNA directed RNA polymerase II 23 kda polypeptide antibody, anti-DNA-directed RNA polymerase II 23 kDa polypeptide antibody, anti-DNA-directed RNA polymerase II subunit E antibody, anti-DNA-directed RNA polymerases I, II, and III subunit RPABC1 antibody, anti-hRPB25 antibody, anti-hsRPB5 antibody, anti-polymerase (RNA) II (DNA directed) polypeptide E (25kD) antibody, anti-polymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibody, anti-RPABC1 antibody, anti-RPB5 antibody, anti-RPB5 homolog antibody, anti-XAP4RNA polymerases I, II, and III subunit ABC1 antibody
7 Results for "polr2e" in Primary Antibodies
![Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - POLR2A is destabilized by inhibition of oncogenic BRAFV600E signaling. Representative immunoblot of A375-P cells treated with PLX4720 or DMSO control for 16 hours, followed by cycoheximide treatment for the indicated timepoints. The lower panel shows the quantification of three independent biological replicates, normalized to the respective 0 CHX timepoint and alpha-Tubulin loading control (mean +/- SD). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-44112-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Western-Blot-NBP2-00482-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - POLR2A is destabilized by inhibition of oncogenic BRAFV600E signaling. Representative immunoblot of A375-P cells treated with PLX4720 or DMSO control for 16 hours, followed by cycoheximide treatment for the indicated timepoints. The lower panel shows the quantification of three independent biological replicates, normalized to the respective 0 CHX timepoint and alpha-Tubulin loading control (mean +/- SD). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-44112-7), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Human tonsil.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0012.jpg)
![Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2E antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Flow-Cytometry-NBP2-00482-img0002.jpg)
![Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Western-Blot-NBP2-00482-img0003.jpg)
![Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - Analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Western-Blot-NBP2-00482-img0011.jpg)
![Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0004.jpg)
![Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0005.jpg)
![Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0006.jpg)
![Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0007.jpg)
![Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human breast tissue using anti-POLR2E mouse monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0008.jpg)
![Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0009.jpg)
![Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody.](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP2-00482-img0010.jpg)
![Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - Analysis of Jurkat cells, using anti-POLR2E antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/POLR2E-Antibody-OTI3B5-Flow-Cytometry-NBP2-00482-img0001.jpg)
![Western Blot: POLR2E Antibody [NBP3-35759] - Western blot analysis of various lysates using POLR2E Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35759_rabbit-polr2e-pab-1012202411552835.jpg "Western Blot: POLR2E Antibody [NBP3-35759] - Western blot analysis of various lysates using POLR2E Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry: POLR2E Antibody [NBP3-35759] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using POLR2E Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35759_rabbit-polr2e-pab-101220241243914.jpg)
![Immunohistochemistry: POLR2E Antibody [NBP3-35759] - Immunohistochemistry analysis of POLR2E in paraffin-embedded rat brain tissue using POLR2E Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35759_rabbit-polr2e-pab-3112025173123.jpg%3Ft=1705995327)
![Immunohistochemistry: POLR2E Antibody [NBP3-35759] - Immunohistochemistry analysis of POLR2E in paraffin-embedded mouse lung tissue using POLR2E Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35759_rabbit-polr2e-pab-3112025170472.jpg%3Ft=1705995327)
![Immunohistochemistry: POLR2E Antibody [NBP3-35759] - Immunohistochemistry analysis of POLR2E in paraffin-embedded human brain tissue using POLR2E Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35759_rabbit-polr2e-pab-311202516593624.jpg%3Ft=1705995327)
![Western Blot: POLR2E Antibody (S05-8J5) [NBP3-14993] - Western blot detection of POLR2E in K562, C6, 3T3, Hela cell lysates using POLR2E Rabbit mAb (1:1000 diluted). Predicted band size: 25kDa. Observed band size: 25kDa.](http://images.novusbio.com/fullsize/POLR2E-Antibody-S05-8J5-Western-Blot-NBP3-14993-img0001.jpg "Western Blot: POLR2E Antibody (S05-8J5) [NBP3-14993] - Western blot detection of POLR2E in K562, C6, 3T3, Hela cell lysates using POLR2E Rabbit mAb (1:1000 diluted). Predicted band size: 25kDa. Observed band size: 25kDa.")
![Immunohistochemistry-Paraffin: POLR2E Antibody (S05-8J5) [NBP3-14993] - Immunohistochemistry of POLR2E in paraffin-embedded Human breast cancer tissue using POLR2E Rabbit mAb at dilution 1/20](http://images.novusbio.com/fullsize/POLR2E-Antibody-S05-8J5-Immunohistochemistry-Paraffin-NBP3-14993-img0002.jpg)
![Western Blot: POLR2E Antibody [NBP2-49577] - Analysis using Anti-POLR2E antibody NBP2-49577 (A) shows similar pattern to independent antibody NBP2-57185 (B).](http://images.novusbio.com/fullsize/POLR2E-Antibody-Western-Blot-NBP2-49577-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: POLR2E Antibody [NBP2-49577] - Analysis using Anti-POLR2E antibody NBP2-49577 (A) shows similar pattern to independent antibody NBP2-57185 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: POLR2E Antibody [NBP2-49577] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/POLR2E-Antibody-Immunohistochemistry-NBP2-49577-img0001.jpg)
![Western Blot: POLR2E Antibody [NBP2-57185] - Analysis using Anti-POLR2E antibody NBP2-57185 (A) shows similar pattern to independent antibody NBP2-49577 (B).](http://images.novusbio.com/fullsize/POLR2E-Antibody-Western-Blot-NBP2-57185-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: POLR2E Antibody [NBP2-57185] - Analysis using Anti-POLR2E antibody NBP2-57185 (A) shows similar pattern to independent antibody NBP2-49577 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: POLR2E Antibody [NBP2-57185] - Staining of human cell line CACO-2 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/POLR2E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57185-img0001.jpg)
![Western Blot: POLR2E Antibody [NBP2-15100] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/POLR2E-Antibody-Western-Blot-NBP2-15100-img0002.jpg "Western Blot: POLR2E Antibody [NBP2-15100] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE, antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: POLR2E Antibody [NBP2-15100] - Paraformaldehyde-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/POLR2E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15100-img0001.jpg)
![Western Blot: POLR2E Antibody [H00005434-B01P] - Analysis of POLR2E expression in transfected 293T cell line by POLR2E polyclonal antibody. Lane 1: POLR2E transfected lysate(23.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/POLR2E-Antibody-Western-Blot-H00005434-B01P-img0002.jpg "Western Blot: POLR2E Antibody [H00005434-B01P] - Analysis of POLR2E expression in transfected 293T cell line by POLR2E polyclonal antibody. Lane 1: POLR2E transfected lysate(23.1 KDa). Lane 2: Non-transfected lysate.")