POLR2E Antibody (OTI3B5) - Azide and BSA Free

Western Blot: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody.

Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-POLR2E antibody, and then analyzed by flow cytometry.

Western Blot: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Human tonsil.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human breast tissue using anti-POLR2E mouse monoclonal antibody.

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody.

Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Analysis of Jurkat cells, using anti-POLR2E antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

POLR2E Antibody (OTI3B5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 24.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for POLR2E Antibody (OTI3B5) - Azide and BSA Free

• DNA directed RNA polymerase II 23 kda polypeptide
• DNA-directed RNA polymerase II 23 kDa polypeptide
• DNA-directed RNA polymerase II subunit E
• DNA-directed RNA polymerases I, II, and III subunit RPABC1
• hRPB25
• hsRPB5
• polymerase (RNA) II (DNA directed) polypeptide E (25kD)
• polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
• RPABC1
• RPB5 homolog
• RPB5
• XAP4RNA polymerases I, II, and III subunit ABC1

Background

POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2E