POLR2E Antibody (OTI3B5) [Janelia Fluor® 525]

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

POLR2E Antibody (OTI3B5) [Janelia Fluor® 525] Summary

• Immunogen: Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for POLR2E Antibody (OTI3B5) [Janelia Fluor® 525]

• DNA directed RNA polymerase II 23 kda polypeptide
• DNA-directed RNA polymerase II 23 kDa polypeptide
• DNA-directed RNA polymerase II subunit E
• DNA-directed RNA polymerases I, II, and III subunit RPABC1
• hRPB25
• hsRPB5
• polymerase (RNA) II (DNA directed) polypeptide E (25kD)
• polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
• RPABC1
• RPB5 homolog
• RPB5
• XAP4RNA polymerases I, II, and III subunit ABC1

Background

POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2E