POLR2C Antibody (3T8K8)

Western Blot: POLR2C Antibody (3T8K8) [NBP3-16871] - Western blot analysis of extracts of various cell lines, using POLR2C antibody (NBP3-16871) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: POLR2C Antibody (3T8K8) [NBP3-16871] - Immunohistochemistry of paraffin-embedded human lung cancer using POLR2C Rabbit mAb (NBP3-16871) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: POLR2C Antibody (3T8K8) [NBP3-16871] - Immunohistochemistry of paraffin-embedded rat lung using POLR2C Rabbit mAb (NBP3-16871) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 3T8K8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

POLR2C Antibody (3T8K8) Summary

• Description: Novus Biologicals Rabbit POLR2C Antibody (3T8K8) (NBP3-16871) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POLR2C (P19387). MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVE
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POLR2C
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POLR2C Antibody (3T8K8)

• DNA-directed RNA polymerase II 33 kDa polypeptide
• DNA-directed RNA polymerase II subunit C
• DNA-directed RNA polymerase II subunit RPB3
• hRPB33
• hsRPB3
• polymerase (RNA) II (DNA directed) polypeptide C (33kD)
• polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
• RNA polymerase II subunit 3
• RNA polymerase II subunit B3
• RPB3
• RPB31
• RPB33

Background

The POLR2C gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2C