Mouse Podoplanin ELISA Kit (Colorimetric)

ELISA: Mouse Podoplanin ELISA Kit (Colorimetric) [NBP2-81235] - Samples were spiked with high concentrations of Mouse Podoplanin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Podoplanin ELISA Kit (Colorimetric) [NBP2-81235] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Mouse Podoplanin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse Podoplanin in samples. No significant cross-reactivity or interference between Mouse Podoplanin and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.78
• Intra-Assay: %CV < 5.68
• Spike Recovery: 90 - 107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PDPN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Podoplanin ELISA Kit (Colorimetric)

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Podoplanin ELISA Kit (NBP2-81235)(1)

Publication using NBP2-81235

• Shirai T, Tsukiji N, Sasaki T et al. Cancer-associated fibroblasts promote venous thrombosis through podoplanin/CLEC-2 interaction in podoplanin-negative lung cancer mouse model Journal of thrombosis and haemostasis : JTH 2023-07-18 [PMID: 37473844] (ELISA)

Bioinformatics

• Gene Symbol: PDPN