Podoplanin Antibody [Unconjugated]

Western Blot shows lysates of MG‑63 human osteosarcoma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/ml of Sheep Anti-Human Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Podoplanin at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Podoplanin was detected in immersion fixed paraffin-embedded sections of human uterus using 2 µg/mL Sheep Anti-Human Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3670) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Podoplanin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Podoplanin
  Glu21-Lys123
  Accession # Q86YL7
• Specificity: Detects human Podoplanin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PDPN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podoplanin Antibody [Unconjugated]

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family (1‑2). Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207‑228) and a short cytoplasmic tail (aa 229‑238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation (2‑3). There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells (4‑5), and numerous tumors, including colorectal tumors (3), squamous cell carcinomas (4, 6), testicular seminoma (7), and brain tumors (8‑10). One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) (2). Their association is dependent on sialic acid on O-glycans of Podoplanin (2). Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis (2). Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth (2).

1. Zimmer, G. et al. (1999) Biochem. J. 341:277.
2. Katsue-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
3. Kato, Y. et al. (2003) J. Biol. Chem. 278:51599.
4. Schacht, V. et al. (2005) Am. J. Pathol. 166:913.
5. Breiteneder-Geleff, S. et al. (1997) Am. J. Pathol. 151:1141.
6. Kato, Y. et al. (2005) Tumour Biol. 26:195.
7. Kato, Y. et al. (2004) Oncogene 23:8552.
8. Mishima, K. et al. (2006) Acta Neuropathol. 111:563.
9. Mishima, K. et al. (2006) Acta Neuropathol. 111:483.
10. Kato, Y. et al. (2006) Biochem. Biophys. Res. Commun. 349:1301.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (AF3670)(20)

Publications using AF3670

• Rouaud, L;Baudin, L;Gautier-Isola, M;Van Meerbeeck, P;Feyereisen, E;Blacher, S;van Baren, N;Kridelka, F;Lucas, S;Noel, A; Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes Cancers 2023-11-28 [PMID: 38067325] (IHC, Human)
• Arooj Munir, Janne Elin Reseland, Hanna Tiainen, Håvard Jostein Haugen, Pawel Sikorski, Emil Frang Christiansen et al. Osteocyte‐Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model JBMR Plus 6/28/2023 [PMID: 37701151]
• K Lim, APA Donovan, W Tang, D Sun, P He, JP Pett, SA Teichmann, JC Marioni, KB Meyer, AH Brand, EL Rawlins Organoid modeling of human fetal lung alveolar development reveals mechanisms of cell fate patterning and neonatal respiratory disease Cell Stem Cell, 2022-12-08;30(1):20-37.e9. 2022-12-08 [PMID: 36493780] (IHC, Human)
• Zhu J, Fan Y, Xiong Y et al. Delineating the dynamic evolution from preneoplasia to invasive lung adenocarcinoma by integrating single-cell RNA sequencing and spatial transcriptomics Experimental & molecular medicine 11/25/2022 [PMID: 36434043]
• H Moriyama, J Endo, M Kataoka, Y Shimanaka, N Kono, Y Sugiura, S Goto, H Kitakata, T Hiraide, N Yoshida, S Isobe, T Yamamoto, K Shirakawa, A Anzai, Y Katsumata, M Suematsu, K Kosaki, K Fukuda, H Arai, M Sano Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling Nature Communications, 2022-05-31;13(1):3013. 2022-05-31 [PMID: 35641514] (IHC, Human)
• TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease Proceedings of the National Academy of Sciences of the United States of America, 2022-03-23;119(13):e2120336119. 2022-03-23 [PMID: 35320046] (CyTof, IHC, Human)
• K Gkatzis, P Panza, S Peruzzo, DY Stainier Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures Elife, 2021-09-29;10(0):. 2021-09-29 [PMID: 34586063] (IHC, Mouse)
• H Kwon, JY Kwon, J Song, YS Maeng Decreased Lymphangiogenic Activities and Genes Expression of Cord Blood Lymphatic Endothelial Progenitor Cells (VEGFR3+/Pod+/CD11b+ Cells) in Patient with Preeclampsia International Journal of Molecular Sciences, 2021-04-19;22(8):. 2021-04-19 [PMID: 33921847] (ICC, Human)
• TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal Cellular census of human fibrosis defines functionally distinct stromal cell types and states Nat Commun, 2020-06-02;11(1):2768. 2020-06-02 [PMID: 32488016] (CyTof, Human)
• Parikh AS, Puram SV, Faquin WC et al. Immunohistochemical quantification of partial-EMT in oral cavity squamous cell carcinoma primary tumors is associated with nodal metastasis Oral Oncol. 11/5/2019 [PMID: 31704557]
• KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis BMC Musculoskelet Disord, 2019-07-27;20(1):344. 2019-07-27 [PMID: 31351471] (IHC-P, Canine, Human)
• SATOMI ARIMOTO, TAKUMI HASEGAWA, DAISUKE TAKEDA, IZUMI SAITO, RIKA AMANO, MASAYA AKASHI et al. Lymphangiogenesis and Lymph Node Metastasis in Oral Squamous Cell Carcinoma Anticancer Research 11/1/2018 [PMID: 30396932]
• Kinchen J, Chen HH, Parikh K et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell 9/25/2018 [PMID: 30270042]
• H Tempfer, A Kaser-Eich, C Lehner, R Gehwolf, S Korntner, N Kunkel, A Wagner, M Gruetz, LM Heindl, F Schroedl, A Traweger Bevacizumab Improves Achilles Tendon Repair in a Rat Model Cell. Physiol. Biochem., 2018-04-16;46(3):1148-1158. 2018-04-16 [PMID: 29672303] (IHC, Rat)
• MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells Nat Commun, 2018-03-05;9(1):948. 2018-03-05 [PMID: 29507342] (ICC, Mouse)
• E Reátegui, KE van der Vo, CP Lai, M Zeinali, NA Atai, B Aldikacti, FP Floyd, A H Khankhel, V Thapar, FH Hochberg, LV Sequist, BV Nahed, B S Carter, M Toner, L Balaj, D T Ting, XO Breakefiel, SL Stott Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles Nat Commun, 2018-01-12;9(1):175. 1/12/2018 [PMID: 29330365]
• Puram Sidharth V, Tirosh Itay, Parikh Anuraag S et al. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell 12/14/2018 [PMID: 29198524]
• Nikoli? MZ, Caritg O, Jeng Q et al. Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids Elife 6/30/2017 [PMID: 28665271]
• LD Volk-Drape, KL Hall, AC Wilber, S Ran Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4 PLoS ONE, 2017-06-09;12(6):e0179257. 2017-06-09 [PMID: 28598999] (Flow Cytometry, Human)
• Jennifer M Munson Quantitative Analysis of the Cellular Microenvironment of Glioblastoma to Develop Predictive Statistical Models of Overall Survival J. Neuropathol. Exp. Neurol., 2016-12-01;0(0):. 2016-12-01 [PMID: 27815396] (IHC-P, Human)

Bioinformatics

• Gene Symbol: PDPN
• Uniprot:
  • Q86YL7()