Podoplanin Antibody (pmab-1)

Images

 
Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) ...read more
Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the PE conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse F9 tumor cell line probed using 0.5 ug of podoplanin antibody (red) ...read more
Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Detection of mouse fetal lung tissue using Podoplanin antibody at a conentration of 1 ug/mL.
Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). ...read more
Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin ...read more
Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
pmab-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Podoplanin Antibody (pmab-1) Summary

Immunogen
Podoplanin
Marker
Lymphatic Endothelium Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
PDPN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3 - 5 ug/mL
  • Flow Cytometry 0.5 ug/10^5 cells
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Podoplanin Antibody (pmab-1)

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin, also known as gp38, is a 43kD type 1 transmembrane glycoprotein found on many tissues. Podoplanin appears to be differentially expressed by lymphatic endothelial cells but not blood vessel endothelial cells (Schacht, 2005). Podoplanin has also been found to be expressed on a wide variety of tumors. It appears to be involved in lymphangioigenesis and promoting cell migration (Farr, 1992). Recently, there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). In a murine EAE model for autoimmune disease it has been shown that Th17 cells migrate quickly to the CNS at EAE onset. Injection of antibody to podoplanin in mice also immunized with myelin antigen to induce EAE results in elevated numbers of Th17 cells in the CNS. Thus as in effecting migration of tumor cells, podoplanin may also promote migration of committed Th17 cells to sites of inflammation in autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-03955AF350
Alexa Fluor 405 NBP2-03955AF405
Alexa Fluor 488 NBP2-03955AF488
Alexa Fluor 532 NBP2-03955AF532
Alexa Fluor 594 NBP2-03955AF594
Alexa Fluor 647 NBP2-03955AF647
Alexa Fluor 700 NBP2-03955AF700
Alexa Fluor 750 NBP2-03955AF750
Allophycocyanin NBP2-03955APC
Biotin NBP2-03955B
DyLight 350 NBP2-03955UV
DyLight 405 NBP2-03955V
DyLight 488 NBP2-03955G
DyLight 550 NBP2-03955R
DyLight 594 NBP2-03955DL594
DyLight 650 NBP2-03955C
DyLight 680 NBP2-03955FR
DyLight 755 NBP2-03955IR
FITC NBP2-03955F
HRP NBP2-03955H
Janelia Fluor 549 NBP2-03955JF549
Janelia Fluor 646 NBP2-03955JF646
PerCP NBP2-03955PCP

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Bioinformatics Tool for Podoplanin Antibody (NBP2-03955)

Discover related pathways, diseases and genes to Podoplanin Antibody (NBP2-03955). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Podoplanin Antibody (NBP2-03955)

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Blogs on Podoplanin.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Bioinformatics

Gene Symbol PDPN
Uniprot
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