Podoplanin Antibody (pmab-1) - BSA Free

Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/mL. Mouse tissue Insta-blot, goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the PE conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse F9 tumor cell line probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). Cell surface flow kit was used for this test.

Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Detection of mouse fetal lung tissue using Podoplanin antibody at a conentration of 1 ug/mL.

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-03955 and APC-conjugated secondary antibody were used for this test.

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse LLC1 cells were probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: pmab-1
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Podoplanin Antibody (pmab-1) - BSA Free Summary

• Immunogen: Podoplanin
• Marker: Lymphatic Endothelium Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: PDPN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5 ug/10^5 cells
  • Western Blot 3 - 5 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Podoplanin Antibody (pmab-1) - BSA Free

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin, also known as gp38, is a 43kD type 1 transmembrane glycoprotein found on many tissues. Podoplanin appears to be differentially expressed by lymphatic endothelial cells but not blood vessel endothelial cells (Schacht, 2005). Podoplanin has also been found to be expressed on a wide variety of tumors. It appears to be involved in lymphangioigenesis and promoting cell migration (Farr, 1992). Recently, there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). In a murine EAE model for autoimmune disease it has been shown that Th17 cells migrate quickly to the CNS at EAE onset. Injection of antibody to podoplanin in mice also immunized with myelin antigen to induce EAE results in elevated numbers of Th17 cells in the CNS. Thus as in effecting migration of tumor cells, podoplanin may also promote migration of committed Th17 cells to sites of inflammation in autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (NBP2-03955)(1)

Publication using NBP2-03955

• Nagre N, Nicholson G, Cong X et al. Activation of cannabinoid-2 receptor protects against Pseudomonas aeruginosa induced acute lung injury and inflammation Respiratory research 2022-12-03 [PMID: 36463179] (IHC-P, Mouse)
  
  Details:
  Dilution used in IHC-P 1:100

Bioinformatics

• Gene Symbol: PDPN
• Uniprot:
  • NP_034459.2(Mouse)