Podocalyxin Like Antibody

Western Blot: Podocalyxin Antibody [NBP2-19880] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Podocalyxin Antibody [NBP2-19880] - Analysis of methanol-fixed mouse ESC, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-19880] - Paraffin-embedded human normal kidney. PODXL antibody [C2C3], C-term diluted at 1:500.

Product Discontinued

Podocalyxin Like Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PODXL. The exact sequence is proprietary.
• Marker: Podocytes /Apical Marker, Endothelial Cell Marker
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PODXL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Protein A purified

Alternate Names for Podocalyxin Like Antibody

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PODXL
• Uniprot:
  • O00592(Human)