HLA DRB1 Antibody (LN-3 + HLA-DRB/1067)

Images

 
Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067).
Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). conjugated with APC (Blue); Isotype Control (Red).
Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Human Spleen tissue lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067).
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Histiocytoma stained with HLA-DR Monoclonal Antibody (LN-3 + HLA-DRB/1067).
Immunofluorescence: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Immunofluorescence Analysis of Raji cells labeling HLA-DR with HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) conjugated with CF594 (Cyan).The nuclear ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P, ICC/IF
Clone
LN-3 + HLA-DRB/1067
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) Summary

Immunogen
Activated human peripheral blood mononuclear cells (LN-3 and HLA-DRB/1067)
Localization
Cell surface
Specificity
This MAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DRB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 6p21.3
Molecular weight of antigen: 28kDa (beta chain)
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Publications
Read Publication using NBP2-45316.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for HLA DRB1 Antibody (LN-3 + HLA-DRB/1067)

  • Clone P2-beta-3
  • DR1
  • DR-1
  • DR12
  • DR-12
  • DR13
  • DR-13
  • DR14
  • DR-14
  • DR16
  • DR-16
  • DR4
  • DR-4
  • DR5
  • DR-5
  • DR7
  • DR-7
  • DR8
  • DR-8
  • DR9
  • DR-9
  • DRB1
  • DRw10
  • DRw11
  • DRw8
  • DW2.2/DR2.2
  • FLJ75017
  • FLJ76359
  • HLA class II antigen beta chain
  • HLA class II histocompatibility antigen, DR-1 beta chain
  • HLA-DR1B
  • HLA-DRB
  • HLA-DRB1*
  • HLA-DRB2
  • HLA-DR-beta 1
  • human leucocyte antigen DRB1
  • leucocyte antigen DR beta 1 chain
  • leucocyte antigen DRB1
  • lymphocyte antigen DRB1
  • major histocompatibility complex, class II, DR beta 1
  • MHC class II antigen DRB1*1
  • MHC class II antigen DRB1*10
  • MHC class II antigen DRB1*11
  • MHC class II antigen DRB1*12
  • MHC class II antigen DRB1*13
  • MHC class II antigen DRB1*14
  • MHC class II antigen DRB1*15
  • MHC class II antigen DRB1*16
  • MHC class II antigen DRB1*3
  • MHC class II antigen DRB1*4
  • MHC class II antigen DRB1*7
  • MHC class II antigen DRB1*8
  • MHC class II antigen DRB1*9
  • MHC class II antigen HLA-DR13
  • MHC class II HLA-DR beta 1 chain
  • MHC class II HLA-DR-beta cell surface glycoprotein
  • MHC class II HLA-DRw10-beta
  • SS1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA DRB1 Antibody (NBP2-45316)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HLA DRB1 Antibody (NBP2-45316). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?
    • While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47672AF350
Alexa Fluor 405 NBP2-47672AF405
Alexa Fluor 488 NBP2-47672AF488
Alexa Fluor 532 NBP2-47672AF532
Alexa Fluor 594 NBP2-47672AF594
Alexa Fluor 647 NBP2-47672AF647
Alexa Fluor 700 NBP2-47672AF700
Alexa Fluor 750 NBP2-47672AF750
Allophycocyanin NBP2-47672APC
Allophycocyanin/Cy7 NBP2-47672APCCY7
Biotin NBP2-47672B
DyLight 350 NBP2-47672UV
DyLight 405 NBP2-47672V
DyLight 488 NBP2-47672G
DyLight 550 NBP2-47672R
DyLight 594 NBP2-47672DL594
DyLight 650 NBP2-47672C
DyLight 680 NBP2-47672FR
DyLight 755 NBP2-47672IR
FITC NBP2-47672F
HRP NBP2-47672H
Janelia Fluor 549 NBP2-47672JF549
Janelia Fluor 646 NBP2-47672JF646
PE NBP2-47672PE
PE/Atto594 NBP2-47672PEATT594
PE/Cy5.5 NBP2-47672PECY55
PE/Cy7 NBP2-47672PECY7
PerCP NBP2-47672PCP

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Bioinformatics

Gene Symbol HLA-DRB1
Uniprot