PLK1 Antibody (2G12)

Images

 
Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of monoclonal antibody to PLK1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - PLK1 monoclonal antibody (M01), clone 2G12 Analysis of PLK1 expression in Hela S3 NE.
Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: ...read more
Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and ...read more
Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal ...read more
ELISA: PLK1 Antibody (2G12) [H00005347-M01] - Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
Clone
2G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PLK1 Antibody (2G12) Summary

Immunogen
PLK1 (AAH02369, 1 a.a. - 603 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS
Localization
Nuclear
Marker
Mitosis Marker
Specificity
PLK1 - polo-like kinase 1 (Drosophila)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PLK1
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Proximity Ligation Assay
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IHC-P usage reported in scientific literature (PMID: 24722282).
Publications
Read Publication using
H00005347-M01 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Ascites

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLK1 Antibody (2G12)

  • cell cycle regulated protein kinase
  • EC 2.7.11
  • EC 2.7.11.21
  • PLK
  • PLK1
  • PLK-1
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase 1polo-like kinase (Drosophila)
  • Serine/threonine-protein kinase 13
  • serine/threonine-protein kinase PLK1
  • STPK13

Background

PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family, cdc5/polo subfamily. PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa. PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK1 is modified by phosphorylation at Threonine 210. PLK1 may also be required for cell division, depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PLK1 Antibody (H00005347-M01)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
IP
(1)
WB
(1)
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Filter By Species
Human
(1)
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Showing Publication 1 - 1 of 1.
Publication using H00005347-M01 Applications Species
Werle K, Chen J, Xu HG et al. Liver kinase B1 regulates the centrosome via PLK1. Cell Death Dis 2014 Apr 11 [PMID: 24722282] (IP, WB, ICC/IF, IHC-P, Human) IP, WB, ICC/IF, IHC-P Human

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PLK1
Entrez
OMIM
Uniprot
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