Plexin A2 Antibody [Allophycocyanin]

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human/Mouse/Rat Plexin A2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5486A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu, Rt
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

Plexin A2 Antibody [Allophycocyanin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2
  Met35-Pro1237
  Accession # P70207
• Specificity: Detects mouse and rat Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A3, and recombinant human Plexin B1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLXNA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin A2 Antibody [Allophycocyanin]

• FLJ11751
• FLJ30634
• KIAA0463plexin 2
• OCT
• OCTSemaphorin receptor OCT
• Plexin A2
• PLXN2
• PLXN2plexin-A2
• PLXNA2
• transmembrane protein OCT

Background

Plexin A2 (also OCT) is a 230-250 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, CD31 + LYVE + lymphoid endothelial cells, osteoblasts, and cardiac neural crest cells. It blocks neurite outgrowth and lymphatic capillary formation, but promotes cell migration and osteogenesis, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilins for Sema3A and C. Notably, it would appear to contribute to the receptor complex for BMPs by interacting with both BMPR1A/ALK3 and BMPR1B/ALK6 in conjunction with BMPR2. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane homodimeric glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin A2 Antibody (FAB5486A)(1)

Publication using FAB5486A

• Barnkob, MB;Michaels, YS;André, V;Macklin, PS;Gileadi, U;Valvo, S;Rei, M;Kulicke, C;Chen, JL;Jain, V;Woodcock, VK;Colin-York, H;Hadjinicolaou, AV;Kong, Y;Mayya, V;Mazet, JM;Mead, GJ;Bull, JA;Rijal, P;Pugh, CW;Townsend, AR;Gérard, A;Olsen, LR;Fritzsche, M;Fulga, TA;Dustin, ML;Jones, EY;Cerundolo, V; Semmaphorin 3?A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells Nature communications 2024-04-12 [PMID: 38609390] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: PLXNA2
• Uniprot:
  • P70207()