Pleckstrin Antibody

Western Blot: Pleckstrin Antibody [NBP1-87370] - Analysis in control (vector only transfected HEK293T lysate) and PLEK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370] - Staining in human bone marrow and pancreas tissues using anti-PLEK antibody. Corresponding PLEK RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370] - Staining of human bone marrow shows high expression.

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370] - Staining of human pancreas shows low expression as expected.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Pleckstrin Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFLDNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLEK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Pleckstrin Protein (NBP1-87370PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Pleckstrin Antibody

• FLJ27168
• p47
• P47Platelet 47 kDa protein
• pleckstrin

Background

Pleckstrin is the major protein kinase C substrate of platelets however its exact function is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLEK