Pleckstrin Antibody - BSA Free

Western Blot: Pleckstrin Antibody [NBP1-31099] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Pleckstrin antibody [C2C3], C-term (NBP1-31099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Pleckstrin antibody [C2C3], C-term detects PLEK protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min.Green: PLEK protein stained by Pleckstrin antibody [C2C3], C-term (NBP1-31099) diluted at 1:500.Blue: Hoechst 33343 staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pleckstrin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Pleckstrin Antibody - BSA Free (NBP1-31099) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Pleckstrin. The exact sequence is proprietary.
• Predicted Species: Mouse (93%), Rat (93%), Canine (93%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLEK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Pleckstrin Antibody - BSA Free

• FLJ27168
• p47
• P47Platelet 47 kDa protein
• pleckstrin

Background

Pleckstrin is the major protein kinase C substrate of platelets however its exact function is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PLEK