PLC-gamma 1 Antibody (S01-6B4)

Western Blot: PLC-gamma 1 Antibody (S01-6B4) [NBP3-20073] - Western blot detection of PLC-gamma 1 in U2OS, C6 using NBP3-20073 (1:1000 diluted)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S01-6B4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

PLC-gamma 1 Antibody (S01-6B4) Summary

• Description: Novus Biologicals Rabbit PLC-gamma 1 Antibody (S01-6B4) (NBP3-20073) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human PLC-gamma 1 (Uniprot # P19174)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLCG1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for PLC-gamma 1 Antibody (S01-6B4)

• EC 3.1.4.11
• gamma 1 (formerly subtype 148)
• monophosphatidylinositol phosphodiesterase
• NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
• phosphatidylinositol phospholipase C
• phosphoinositidase C
• phosphoinositide phospholipase C
• Phosphoinositide phospholipase C-gamma-1
• phospholipase C, gamma 1
• phospholipase C-148
• Phospholipase C-gamma-1
• Phospholipase C-II
• PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
• PLC148
• PLC-148
• PLCG1
• PLCgamma 1
• PLC-gamma 1
• PLC-gamma-1
• PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• PPLCA
• triphosphoinositide phosphodiesterase

Background

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCG1