PLC-beta 4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PLC-beta 4 Antibody [NBP2-58912] - Staining of human cell line U-251 MG shows localization to microtubules. Antibody staining is shown in green.

PLC-beta 4 Antibody [NBP2-58912] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

PLC-beta 4 Antibody [NBP2-58912]- Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

PLC-beta 4 Antibody [NBP2-58912] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

PLC-beta 4 Antibody [NBP2-58912] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PLC-beta 4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALASGVEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKL
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLCB4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-P Retrieval method: HIER pH6.
• Control Peptide:

  PLC-beta 4 Recombinant Protein Antigen (NBP2-58912PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PLC-beta 4 Antibody - BSA Free

• 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 4
• 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4
• beta 4 (1-Phosphatidylinositol-4,5-BisphosphatePhosphodiesterase Beta 4))
• EC 3.1.4.11
• FLJ16169
• inositoltrisphosphohydrolase
• monophosphatidylinositol phosphodiesterase
• phosphoinositidase C
• phosphoinositide phospholipase C
• phospholipase C, beta 4
• PLCB4
• PLCbeta 4
• PLC-beta 4
• triphosphoinositide phosphodiesterase

Background

The protein encoded by the Phospholipase C beta 4 gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals in the retina. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCB4