PKMYT1 Antibody (2A3) - Azide and BSA Free

Sandwich ELISA: PKMYT1 Antibody (2A3) [H00009088-M03] - Detection limit for recombinant GST tagged PKMYT1 is 1 ng/ml as a capture antibody.

Proximity Ligation Assay: PKMYT1 Antibody (2A3) [H00009088-M03] - Analysis of protein-protein interactions between CCNB1 and PKMYT1. Huh7 cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PKMYT1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: WB, ELISA, Func
• Clone: 2A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKMYT1 Antibody (2A3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PKMYT1 Antibody (2A3) - Azide and BSA Free (H00009088-M03) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PKMYT1 (NP_004194.3, 400 a.a. ~ 499 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT
• Specificity: PKMYT1 - protein kinase, membrane associated tyrosine/threonine 1 (2A3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKMYT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKMYT1 Antibody (2A3) - Azide and BSA Free

• DKFZp547K1610
• EC 2.7.11.1
• FLJ20093
• Myt1 kinase
• MYT1membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• protein kinase, membrane associated tyrosine/threonine 1

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKMYT1
• Entrez:
  • 9088(Human)
• Uniprot:
  • NP_004194(Human)