PKM2 Antibody [Biotin]

Western Blot: PKM2 Antibody [Biotin] [NB120-20589] - Western Blot of PKM2 antibody [Biotin].Lane 1: Opal Prestain Molecular Weight Marker.Lane 2: 50ng of PKM2.Primary Antibody: Goat Anti-PKM2 Biotin at 1ug/mL o/n @ 4C.Secondary Antibody: Streptavidin-HRP: S000-03 at 1:40,000 for 30 min at RT.Blocking: 30 min at RT.

• Reactivity: Rb
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

PKM2 Antibody [Biotin] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]
• Immunogen: PKM2 [Rabbit Muscle] (Uniprot: P11974)
• Localization: Cytoplasmic
• Specificity: This product has been reported to react with all forms of pyruvate kinase (pan M-PK).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PKM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Western Blot 1:200-1:1000
• Application Notes: This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Cross reactivity against PKM2 from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for PKM2 Antibody [Biotin]

• CTHBP
• Cytosolic thyroid hormone-binding protein
• MGC3932
• OIP3
• OIP-3
• OIP3EC 2.7.1.40
• Opa-interacting protein 3
• p58
• PK, muscle type
• PK2
• PK3
• PK3PKM
• PKM2
• Pyruvate kinase 2/3
• pyruvate kinase isozymes M1/M2
• Pyruvate kinase muscle isozyme
• pyruvate kinase, muscle
• TCB
• THBP1
• THBP1p58
• Thyroid hormone-binding protein 1
• thyroid hormone-binding protein, cytosolic
• Tumor M2-PK

Background

Pyruvate kinase is also known as ATP:pyruvate phosphotransferase. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney. PK1 is found also in liver. During fetal development, PK3 changes to PK1 in the liver. PK1 is a tetramer composed of two dissimilar polypeptides of somewhat different molecular weight. It is an allosteric enzyme exhibiting cooperative binding for phosphoenolpyruvate and sensitivity to fructose-1,6-diphosphate. PK3 also is a tetrameric protein but, unlike PK1, all subunits are alike and, not unexpectedly, there is no cooperative behavior. The enzyme is insensitive to fructose-1,6-diphosphate. Patients with deficiency of red cell PK have normal PK2 and PK3. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. Ab38237 recognizes the M1/M2 isoform of pyruvate kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKM
• Uniprot:
  • P11974(Rabbit)