PKM2 Antibody - BSA Free

• Reactivity: Rb
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

PKM2 Antibody - BSA Free Summary

• Description: This antibody immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyruvate Kinase [Rabbit Muscle]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: PKM2 [Rabbit Muscle] (Uniprot: P11974)
• Localization: Cytoplasmic
• Specificity: This product has been reported to react with all forms of pyruvate kinase (pan M-PK).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PKM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:50000-1:200000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:5000-1:20000
• Application Notes: This product is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Specific conditions should be optimized by user.

Reactivity Notes

Cross reactivity against PKM2 from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: IgG purified

Alternate Names for PKM2 Antibody - BSA Free

• CTHBP
• Cytosolic thyroid hormone-binding protein
• MGC3932
• OIP3
• OIP-3
• OIP3EC 2.7.1.40
• Opa-interacting protein 3
• p58
• PK, muscle type
• PK2
• PK3
• PK3PKM
• PKM2
• Pyruvate kinase 2/3
• pyruvate kinase isozymes M1/M2
• Pyruvate kinase muscle isozyme
• pyruvate kinase, muscle
• TCB
• THBP1
• THBP1p58
• Thyroid hormone-binding protein 1
• thyroid hormone-binding protein, cytosolic
• Tumor M2-PK

Background

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKM2 Antibody (NB600-1447)(1)

Publication using NB600-1447

• Jindal HK, Merchant E, Balschi JA et al. Proteomic analyses of transgenic LQT1 and LQT2 rabbit hearts elucidate an increase in expression and activity of energy producing enzymes J Proteomics 2012-07-14 [PMID: 22796357] (WB, Rabbit)

Bioinformatics

• Gene Symbol: PKM
• Uniprot:
  • P11974(Rabbit)