PKC theta Antibody - BSA Free

Immunoprecipitation:[NBP3-29620]-Detection of human PRKCQ by western blot of immunoprecipitates. Samples: Whole cell lysate from Jurkat cells. Antibodies: Affinity purified rabbit anti-PRKCQ antibody used for IP at 6 µg/mg lysate. PRKCQ was also immunoprecipitated by rabbit anti-PRKCQ antibody. For blotting immunoprecipitated PRKCQ, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKC theta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PKC theta Antibody - BSA Free (NBP3-29620) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 350 and 400
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCQ
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for PKC theta Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• MGC126514
• MGC141919
• nPKC-theta
• PKC theta
• PRKCQ
• PRKCT
• protein kinase C theta type
• protein kinase C, theta

Background

Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca++. On the other hand, nPKC and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCQ