MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox">

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." alt="Jurkat human acute T cell leukemia cell line was stained with Human PKC theta Monoclonal Antibody (Catalog # MAB4368, filled histogram) or isotype control antibody (Catalog #
MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_thumb" />
HAF018). A specific band was detected for PKC theta at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">

HAF018). A specific band was detected for PKC theta at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />