PKC alpha Antibody (MC5)

Images

 
Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha detected in muse prostate tissue using PKC alpha Antibody (MC5). IHC-P image submitted by a verified customer review.
Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # ...read more
Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fi, Pm, Pm, Rb, Sh, ZeSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC, IP
Clone
MC5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PKC alpha Antibody (MC5) Summary

Immunogen
Purified bovine brain Protein Kinase C
Specificity
This antibody is capable of detecting PKC beta as well.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRKCA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation 1:10-1:100
  • Radioimmunoassay
  • Western Blot 1:1000
Application Notes
This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
Reviewed Applications
Read 1 Review rated 5
using
NB600-201 in the following applications:

Publications
Read Publications using
NB600-201 in the following applications:

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589) Use in Baboon reported in scientific literature (PMID:34314739) Zebrafish reactivity reported in scientific literature (PMID: 31273575)..

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PKC alpha Antibody (MC5)

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC 2.7.11.13
  • MGC129901
  • PKC alpha
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • PRKACA
  • PRKCA
  • protein kinase C alpha type
  • protein kinase C, alpha

Background

Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-19846
Species: Hu, Rt, Xp
Applications: Func, ICC/IF, IHC, IHC-P, WB
MAB1230
Species: Hu, Mu, Rt
Applications: ICC, IHC, KO, Simple Western, WB
MAB6666
Species: Hu
Applications: WB
NBP2-03620
Species: Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-90351
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP1-32535
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
236-EG
Species: Hu
Applications: BA
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
AF1347
Species: Hu, Mu, Rt
Applications: ICC, IHC, Simple Western, WB
MAB4540
Species: Hu, Mu, Rt
Applications: IHC, WB

Publications for PKC alpha Antibody (NB600-201)(27)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Monkey, Zebrafish.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(4)
IF/IHC
(3)
IHC-Fr
(1)
IHC-P
(2)
IP
(1)
WB
(11)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
Rat
(7)
Chicken
(1)
Monkey
(1)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB600-201 Applications Species
Bernardo-Col�n A, Dong L, Abu-Asab M et al. Ablation of Pigment Epithelium-derived Factor Receptor (PEDF-R/Pnpla2) Causes Photoreceptor Degeneration Journal of lipid research 2023-03-17 [PMID: 36934843] (IHC-P, Mouse)

Details:
Dilution: 1:50
IHC-P Mouse
Ren J, Hu Z, Li Q et al. Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-?B-IRF1 signaling BMC biology 2023-03-08 [PMID: 36882743] (ICC/IF, Chicken) ICC/IF Chicken
Antoniali G, Dalla E, Mangiapane G et al. APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b Cellular and molecular life sciences : CMLS 2022-07-25 [PMID: 35876890] (WB) WB
Yamasaki S, Tu H, Matsuyama T et al. A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation iScience 2022-01-01 [PMID: 35024589] (IHC-Fr, Monkey) IHC-Fr Monkey
Xie Z, Wang Q, Hu S. Coordination of PRKCA/PRKCA-AS1 interplay facilitates DNA methyltransferase 1 recruitment on DNA methylation to affect protein kinase C alpha transcription in mitral valve of rheumatic heart disease Bioengineered 2021-09-06 [PMID: 34482802]
Matsuyama T, Tu HY, Sun J Et al. Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation iScience 2021-08-20 [PMID: 34409267]
Della Santina L, Yu A, Harris S, et al. Disassembly and Rewiring of a Mature Converging Excitatory Circuit Following Injury Cell Rep 2021-08-04 [PMID: 34348156]
Chen Aw, Biggar K, Nygard K Et Al. IGFBP-1 hyperphosphorylation in response to nutrient deprivation is mediated by activation of protein kinase Calpha (PKCalpha) Molecular and cellular endocrinology 2021-07-24 [PMID: 34314739] (IHC-P, WB) IHC-P, WB
Thomson B, Grannonico M, Liu F et Al. Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma Transl Vis Sci Technol 2020-08-21 [PMID: 32818103]
Grillo SL, McDevitt DS, Voas MG et al. Adenosine receptor expression in the adult zebrafish retina Purinergic Signal 2019-07-06 [PMID: 31273575] (IF/IHC, Zebrafish) IF/IHC Zebrafish
Show All 27 Publications.

Review for PKC alpha Antibody (NB600-201) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-201:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PKC alpha NB600-201
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 07/30/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedprostate
SpeciesMouse
Lot255

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKC alpha Antibody (NB600-201). (Showing 1 - 4 of 4 FAQs).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.
  2. I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research. I'd like to know how many antibody kit I'll need for my sample (approx. 90 specimens).
    • Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.
  3. I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research, can you provide me with the protocol? I can't access the two publications stated on your website for the protocol.
    • We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.
  4. In our data base it is stated as PKC pan, on your data sheet is stated PKC alpha. Could you please confirm, if it is really PKC alpha, because from other suppliers this clone is stated for detecting other isoforms from PKC as well and in previous product lists from Novus, it is stated as "pan" as well. It would be very important, because the customer needs a product, which detects PKC pan and not only alpha.
    • According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

Secondary Antibodies

 

Isotype Controls

Additional PKC alpha Products

Diseases for PKC alpha Antibody (NB600-201)

Discover more about diseases related to PKC alpha Antibody (NB600-201).
 

Pathways for PKC alpha Antibody (NB600-201)

View related products by pathway.

PTMs for PKC alpha Antibody (NB600-201)

Learn more about PTMs related to PKC alpha Antibody (NB600-201).
 

Research Areas for PKC alpha Antibody (NB600-201)

Find related products by research area.

Blogs on PKC alpha.

There is nothing beta than PKC Alpha
cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
07/30/2021
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol PRKCA
Entrez
Uniprot