Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.
Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # ...read more
This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
Human, mouse, rat, bovine, sheep, rabbit, canine, porcine and fish. Chicken reactivity reported in scientific literature (PMID: 26719241).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Protein A purified
Alternate Names for PKC alpha Antibody (MC5)
aging-associated gene 6
protein kinase C alpha type
protein kinase C, alpha
Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for PKC alpha Antibody (NB600-201). (Showing 1 - 4 of 4 FAQs).
We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.
I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research. I'd like to know how many antibody kit I'll need for my sample (approx. 90 specimens).
Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.
I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research, can you provide me with the protocol? I can't access the two publications stated on your website for the protocol.
We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.
In our data base it is stated as PKC pan, on your data sheet is stated PKC alpha. Could you please confirm, if it is really PKC alpha, because from other suppliers this clone is stated for detecting other isoforms from PKC as well and in previous product lists from Novus, it is stated as "pan" as well. It would be very important, because the customer needs a product, which detects PKC pan and not only alpha.
According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.
There is nothing beta than PKC Alpha cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel... Read full blog post.
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