PKC alpha Antibody (MC5)

Images

 
Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.
Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fi, Rb, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
Clone
MC5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PKC alpha Antibody (MC5) Summary

Immunogen
Purified bovine brain Protein Kinase C
Specificity
This antibody is capable of detecting PKC beta as well.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRKCA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation 1:10-1:100
  • Radioimmunoassay
Application Notes
This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
Publications
Read Publications using
NB600-201 in the following applications:

  • 3 publications
  • IHC
    3 publications
  • IP
    1 publication
  • WB
    9 publications

Reactivity Notes

Human, mouse, rat, bovine, sheep, rabbit, canine, porcine and fish. Chicken reactivity reported in scientific literature (PMID: 26719241).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PKC alpha Antibody (MC5)

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC 2.7.11.13
  • MGC129901
  • PKC alpha
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • PRKACA
  • PRKCA
  • protein kinase C alpha type
  • protein kinase C, alpha

Background

Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKC alpha Antibody (NB600-201)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(3)
IHC
(3)
IP
(1)
WB
(9)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Rat
(7)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB600-201 Applications Species
Grillo SL, McDevitt DS, Voas MG et al. Adenosine receptor expression in the adult zebrafish retina Purinergic Signal. Jul 4 2019 [PMID: 31273575] (IHC, Zebrafish) IHC Zebrafish
Hellmer CB, Clemons MR, Nawy S, Ichinose T. A group I metabotropic glutamate receptor controls synaptic gain between rods and rod bipolar cells in the mouse retina. Physiol Rep. Oct 1 2018 [PMID: 30338673] (IHC, Mouse) IHC Mouse
Neuhauss S, Haug M, Gesemann M, Berger M. Differential expression of PKCa and -B in the zebrafish retina. bioRxiv Jul 3 2018 (IHC, Zebrafish) IHC Zebrafish
Lin YJ, Liang WM, Chen CJ et al. Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells Phytomedicine Mar 13 2019 [PMID: 30901663] (WB, Human) WB Human
Xie J, Huo S, Li Y et al. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Muller Cell Notch Signaling Pathway Cell Transplant. 2017 Jun 09 [PMID: 28185609] (ICC/IF, Rat) ICC/IF Rat
Guimaraes-Souza EM, Perche O, Morgans CW et al. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Exp Eye Res 2016 May [PMID: 26719241]
Zimov S, Yazulla S. Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells. Cell Tissue Res 2008 Jul [PMID: 18449566]
Quraishi S, Gayet J, Morgans CW et al. Distribution of group-III metabotropic glutamate receptors in the retina. J Comp Neurol;501(6):931-43. 2007 Apr 20. [PMID: 17311335]
Bayley PR, Morgans CW. Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina. J Comp Neurol;500(2):286-98. 2007 Jan 10. [PMID: 17111373]
Ng T, Parsons M, Hughes WE, et al. Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility. EMBO J. 2001 Jun 1 [PMID: 11387207] (ICC/IF, IP, WB, Human) ICC/IF, IP, WB Human
Show All 18 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKC alpha Antibody (NB600-201). (Showing 1 - 4 of 4 FAQs).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.
  2. I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research. I'd like to know how many antibody kit I'll need for my sample (approx. 90 specimens).
    • Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.
  3. I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research, can you provide me with the protocol? I can't access the two publications stated on your website for the protocol.
    • We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.
  4. In our data base it is stated as PKC pan, on your data sheet is stated PKC alpha. Could you please confirm, if it is really PKC alpha, because from other suppliers this clone is stated for detecting other isoforms from PKC as well and in previous product lists from Novus, it is stated as "pan" as well. It would be very important, because the customer needs a product, which detects PKC pan and not only alpha.
    • According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-201AF350
Alexa Fluor 405 NB600-201AF405
Alexa Fluor 488 NB600-201AF488
Alexa Fluor 532 NB600-201AF532
Alexa Fluor 594 NB600-201AF594
Alexa Fluor 647 NB600-201AF647
Alexa Fluor 700 NB600-201AF700
Alexa Fluor 750 NB600-201AF750
Allophycocyanin NB600-201APC
Allophycocyanin/Cy7 NB600-201APCCY7
Biotin NB600-201B
DyLight 350 NB600-201UV
DyLight 405 NB600-201V
DyLight 488 NB600-201G
DyLight 550 NB600-201R
DyLight 594 NB600-201DL594
DyLight 650 NB600-201C
DyLight 680 NB600-201FR
DyLight 755 NB600-201IR
FITC NB600-201F
HRP NB600-201H
Janelia Fluor 549 NB600-201JF549
Janelia Fluor 646 NB600-201JF646
PE NB600-201PE
PE/Atto594 NB600-201PEATT594
PE/Cy5.5 NB600-201PECY55
PE/Cy7 NB600-201PECY7
PerCP NB600-201PCP

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Bioinformatics Tool for PKC alpha Antibody (NB600-201)

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Blogs on PKC alpha.

There is nothing beta than PKC Alpha
cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel...  Read full blog post.

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Bioinformatics

Gene Symbol PRKCA
Entrez
Uniprot