PKC alpha Antibody (MC5)


Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) more
Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ca, Fi, Rb, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
1.0 mg/ml

PKC alpha Antibody (MC5) Summary

Purified bovine brain Protein Kinase C
This antibody is capable of detecting PKC beta as well.
Protein A purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein A purified


Application Notes
This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
Read Publications using
NB600-201 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    8 publications

Reactivity Notes

Human, mouse, rat, bovine, sheep, rabbit, canine, porcine and fish.

Alternate Names for PKC alpha Antibody (MC5)

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC
  • MGC129901
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • protein kinase C alpha type
  • protein kinase C, alpha


Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu, Rt
Applications: WB, Func, ICC/IF, IHC, IHC-P
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Mu
Applications: WB, Flow, IHC, ICC
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Applications: Func-Inh
Species: Hu, Mu, Rt
Applications: WB, Simple Western
Species: Hu, Mu, Rt
Applications: WB, IHC
Species: Hu, Mu, Rt, Po, Bv, Ca, Fi, Rb, Sh
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA

Publications for PKC alpha Antibody (NB600-201)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB600-201 Applications Species
Zimov S, Yazulla S. Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells. Cell Tissue Res 2008 Jul [PMID:18449566]
Quraishi S, Gayet J, Morgans CW et al. Distribution of group-III metabotropic glutamate receptors in the retina. J Comp Neurol;501(6):931-43. 2007 Apr 20. [PMID:17311335]
Bayley PR, Morgans CW. Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina. J Comp Neurol;500(2):286-98. 2007 Jan 10. [PMID:17111373]
Ng T, Parsons M, Hughes WE, et al. Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility. EMBO J. 2001 Jun 1 [PMID:11387207] (ICC/IF, IP, WB, Human) ICC/IF, IP, WB Human
Cesare P, Dekker LV, Sardini A et al. pecific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat. Neuron. 1999 Jul [PMID:10433272] (WB, Rat) WB Rat
Ricciarelli R, Tasinato A, Clement S et al. alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state. Biochem J. 1998 Aug 15 [PMID:9693126] (WB, Rat) WB Rat
Gatti A, Robinson PJ.. Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon. Eur J Biochem. 1997 Oct 1 [PMID:9363758] (WB, Rat) WB Rat
Clement S, Tasinato A, Boscoboinik d et al. The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation. Eur J Biochem. 1997 Jun 15 [PMID:9219534] (WB, Rat) WB Rat
Gatti A, Robinson PJ.. Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation. J Biol Chem. 1996 Dec 6 [PMID:8940195] (WB, Rat) WB Rat
Wyss-Coray T, Masliah E, Toggas SM et al. Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis. J Clin Invest. 1996 Feb 1 [PMID:8609236] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Show All 12 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKC alpha Antibody (NB600-201). (Showing 1 - 4 of 4 FAQs).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.
  2. I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research. I'd like to know how many antibody kit I'll need for my sample (approx. 90 specimens).
    • Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.
  3. I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research, can you provide me with the protocol? I can't access the two publications stated on your website for the protocol.
    • We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.
  4. In our data base it is stated as PKC pan, on your data sheet is stated PKC alpha. Could you please confirm, if it is really PKC alpha, because from other suppliers this clone is stated for detecting other isoforms from PKC as well and in previous product lists from Novus, it is stated as "pan" as well. It would be very important, because the customer needs a product, which detects PKC pan and not only alpha.
    • According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-201AF405
Alexa Fluor (R) 488 Labeled NB600-201AF488
Alexa Fluor (R) 647 Labeled NB600-201AF647
Alexa Fluor (R) 700 Labeled NB600-201AF700
Allophycocyanin Labeled NB600-201APC
DyLight 350 Labeled NB600-201UV
DyLight 405 Labeled NB600-201V
DyLight 405LS Labeled NB600-201V3
DyLight 488 Labeled NB600-201G
DyLight 680 Labeled NB600-201FR
DyLight 755 Labeled NB600-201IR
FITC Labeled NB600-201F
PE Labeled NB600-201PE
PerCP Labeled NB600-201PCP

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Blogs on PKC alpha.

There is nothing beta than PKC Alpha
cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel...  Read full blog post.

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Product PDFs


Gene Symbol PRKCA

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