PKC theta [p Thr538] Antibody - Azide and BSA Free

Western Blot: PKC theta [p Thr538] Antibody [NBP3-05454] - Western blot analysis of extracts of Jurkat and HeLa cells, using PKC theta antibody (NBP3-05454) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes. Hela cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKC theta [p Thr538] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit PKC theta [p Thr538] Antibody - Azide and BSA Free (NBP3-05454) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around T538 of human PRKCQ (NP_006248.1). TNTFC
• Modification: p Thr538
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCQ
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for PKC theta [p Thr538] Antibody - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.13
• MGC126514
• MGC141919
• nPKC-theta
• PKC theta
• PRKCQ
• PRKCT
• protein kinase C theta type
• protein kinase C, theta

Background

Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca++. On the other hand, nPKC and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: PRKCQ