PKC theta [p Ser676] Antibody - BSA Free

Western Blot: PKC theta [p Ser676] Antibody [NB100-82145] - Analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), usingPKC theta [p Ser676] Antibody.

Immunocytochemistry/Immunofluorescence: PKC theta [p Ser676] Antibody [NB100-82145] - Staining of methanol-fixed Hela cells using NB100-82145

Western Blot: PKC theta [p Ser676] Antibody [NB100-82145] - analysis of extracts from PC12 cells untreated or treated with PMA using PKC theta (Phospho-Ser676) Antibody.

Immunohistochemistry-Paraffin: PKC theta [p Ser676] Antibody [NB100-82145] - Staining of human lung carcinoma tissue, using NB100-82145(left) or the same antibody preincubated with blocking peptide(right).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PKC theta [p Ser676] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PKC theta [p Ser676] Antibody - BSA Free (NB100-82145) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKC theta.
• Modification: p Ser676
• Specificity: Detects endogenous levels of PKC theta only when phosphorylated at serine 676.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCQ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PKC theta [p Ser676] Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• MGC126514
• MGC141919
• nPKC-theta
• PKC theta
• PRKCQ
• PRKCT
• protein kinase C theta type
• protein kinase C, theta

Background

Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (alpha, beta, and gamma) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation; 2) novel PKC (sigma, epsilon, eta, and theta) activated independent of calcium; and 3) Atypical PKC (zeta and lamda), which are independent of both calcium and DG. High-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver. PKC theta is a autophosphorylated protein kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCQ
• Entrez:
  • 5588(Human)
  • 18761(Mouse)
  • 85420(Rat)
• Uniprot:
  • Q04759()